Cascade reaction and FRET-based fluorescent probe for the colorimetric and ratiometric signaling of hydrogen sulfide

被引：15

作者：

Huang, Kunzhu ^{[1
]}

Liu, Meihui ^{[1
]}

Wang, Xiaobo ^{[1
]}

Cao, Dongsheng ^{[1
]}

Gao, Feng ^{[2
,3
]}

Zhou, Kechao ^{[2
]}

Wang, Wei ^{[3
]}

Zeng, Wenbin ^{[1
]}

机构：

[1] Cent S Univ, Sch Pharmaceut Sci, Changsha 410013, Hunan, Peoples R China

[2] Cent S Univ, Powder Met Res Inst, Changsha 410013, Hunan, Peoples R China

[3] Cent S Univ, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China

来源：

TETRAHEDRON LETTERS | 2015年 / 56卷 / 24期

基金：

中国国家自然科学基金; 国家教育部博士点专项基金资助;

关键词：

Hydrogen sulfide; Cascade reaction and FRET; Colorimetric and ratiometric fluorescent probe; Live cells' imaging; H2S; CELLS;

D O I：

10.1016/j.tetlet.2015.04.054

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A novel addition-elimination cascade reaction and FRET-based fluorescent probe for the colorimetric and ratiometric signaling of hydrogen sulfide was designed. Employing of 2-formylbenzoic acid as the trapper, the probe was highly selective and sensitive to H2S over other biologically relevant species to give color change from colorless to bright orange for naked eye observation. A linear response to H2S in the range of 0-200 mu M indicated that it could detect H2S quantitatively with a detection limit as low as 0.39 AM. Furthermore, fluorescent inverted microscope imaging experiments demonstrated that the probe could assess intracellular H2S level change. (C) 2015 Elsevier Ltd. All rights reserved,

引用

页码：3769 / 3773

页数：5

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