Site-specific incorporation of 5-methylaminomethyl-2-thiouridine and 2-thiouridine(s) into RNA sequences

被引：8
|
作者：
Leszczynska, Grazyna [1 ]
Pieta, Jakub [1 ]
Leonczak, Piotr [1 ]
Tomaszewska, Agnieszka [2 ]
Malkiewicz, Andrzej [1 ]
机构：
[1] Tech Univ Lodz, Inst Organ Chem, PL-90924 Lodz, Poland
[2] Polish Acad Sci, Ctr Mol & Macromol Studies, PL-90363 Lodz, Poland
来源：
TETRAHEDRON LETTERS | 2012年 / 53卷 / 10期
关键词：
tRNAs modified components; Solid-phase synthesis; Oligoribonucleotides; 2-Thiouridine (s(2)U); 5-Methylaminomethyl-2-thiouridine (mnm(5)s(2)U); 2-Methyladenosine (m(2)A); ANTICODON-DOMAIN; 2-THIOPYRIMIDINE NUCLEOSIDES; PHOSPHORAMIDITE CHEMISTRY; MODIFIED NUCLEOTIDES; WOBBLE URIDINES; BASE PROTECTION; RECOGNITION; PURINE; DESULFURIZATION; 5-SUBSTITUENTS;
D O I：
10.1016/j.tetlet.2011.12.079
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
We present a reproducible protocol for the site-specific incorporation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) into a model RNA fragment and, together with 2-methyladenosine (m(2)A), into the native sequence of the Escherichia coli tRNA(Glu2) anticodon arm (E. coli ASL(Glu2)). This approach is also utilized for the synthesis of oligomers modified with multiple 2-thiouridines. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1214 / 1217
页数：4
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