Site-specific incorporation of 5-methylaminomethyl-2-thiouridine and 2-thiouridine(s) into RNA sequences

被引：8

作者：

Leszczynska, Grazyna ^{[1
]}

Pieta, Jakub ^{[1
]}

Leonczak, Piotr ^{[1
]}

Tomaszewska, Agnieszka ^{[2
]}

Malkiewicz, Andrzej ^{[1
]}

机构：

[1] Tech Univ Lodz, Inst Organ Chem, PL-90924 Lodz, Poland

[2] Polish Acad Sci, Ctr Mol & Macromol Studies, PL-90363 Lodz, Poland

来源：

TETRAHEDRON LETTERS | 2012年 / 53卷 / 10期

关键词：

tRNAs modified components; Solid-phase synthesis; Oligoribonucleotides; 2-Thiouridine (s(2)U); 5-Methylaminomethyl-2-thiouridine (mnm(5)s(2)U); 2-Methyladenosine (m(2)A); ANTICODON-DOMAIN; 2-THIOPYRIMIDINE NUCLEOSIDES; PHOSPHORAMIDITE CHEMISTRY; MODIFIED NUCLEOTIDES; WOBBLE URIDINES; BASE PROTECTION; RECOGNITION; PURINE; DESULFURIZATION; 5-SUBSTITUENTS;

D O I：

10.1016/j.tetlet.2011.12.079

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We present a reproducible protocol for the site-specific incorporation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) into a model RNA fragment and, together with 2-methyladenosine (m(2)A), into the native sequence of the Escherichia coli tRNA(Glu2) anticodon arm (E. coli ASL(Glu2)). This approach is also utilized for the synthesis of oligomers modified with multiple 2-thiouridines. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1214 / 1217

页数：4

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