Dysbiosis of Cervical and Vaginal Microbiota Associated With Cervical Intraepithelial Neoplasia

被引:18
|
作者
Lin, Suibin [1 ]
Zhang, Bin [2 ]
Lin, Yixia [1 ]
Lin, Yueping [1 ]
Zuo, Xiaoyu [3 ]
机构
[1] Zhangpu Hosp, Dept Obstet & Gynecol, Zhangzhou, Peoples R China
[2] First Affiliated Hosp Fujian Med Univ, Dept Obstet & Gynecol, Fuzhou, Peoples R China
[3] Guangzhou Med Univ, Guangzhou Inst Pediat, Guangzhou Women & Childrens Med Ctr, Dept Pediat Surg, Guangzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
cervical intraepithelial neoplasia; dysbiosis; cervicovaginal microbiota; 16S rRNA; microbial migration; HUMAN-PAPILLOMAVIRUS;
D O I
10.3389/fcimb.2022.767693
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Cervical intraepithelial neoplasia (CIN) is a precancerous condition inducing local lesions on the surface of the squamocolumnar junction of the cervix. Despite the role of vaginal microbiota having been under-discussed, the role of the cervical microbiome and the microbial migration across the reproductive tract involved in CIN was limitedly studied. We aimed to synchronously characterize the dysbiosis associated with CIN in both the cervix and vagina in a Chinese population. Profiling of cervical and vaginal microbiota from 60 CIN women and 60 healthy women was conducted. 16S rRNA sequencing was adopted. By comparing the microbial profiles between different parts of the reproductive tract, our results demonstrated an increased shift of microbial diversity in the cervix compared with that in the vagina for the CIN patients, specifically in CIN 1. Less dysbiosis was found between the CIN patients and controls, in either the vagina or cervix. The microbial community may be modulated by the onset of sexual activity, a known clinical risk factor for cervical neoplasia. Distinct patterns of perturbated bacteria were found in the vaginal and cervical microbiota, in which reduced Actinobacteria-related operational taxonomic units (OTUs) and increased Proteobacteria-related OTUs were found in the vagina and cervix, respectively. A good agreement between the direction of the top-significant perturbated OTUs was observed between the vaginal and cervical microbiome, suggesting a potential microbial migration in the reproductive tract. Enriched genera such as Sphingomonas and Stenotrophomonas were found in cervical microbiota-associated CIN. Multivariate analysis revealed Comamonas, Rhizobium, and Pseudomonas as independent genera contributing to CIN in the cervix. In summary, this study revealed the perturbation of microbiota in the presence of CIN and demonstrated a distinct pattern of characteristic bacteria community between the vagina and cervix involved in the development of CIN.
引用
收藏
页数:9
相关论文
共 50 条
  • [1] THE VAGINAL MICROBIOTA AFTER EXCISIONAL TREATMENT FOR CERVICAL INTRAEPITHELIAL NEOPLASIA
    Mitra, A.
    MacIntyre, D.
    Lee, Y.
    Smith, A.
    Marchesi, J.
    Lyons, D.
    Bennett, P.
    Kyrgiou, M.
    INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2017, 27 : 979 - 979
  • [2] Clinical analysis of cervical intraepithelial neoplasia with vaginal intraepithelial neoplasia
    He, Yue
    Zhao, Qun
    Geng, Yu-Ning
    Yang, Shu-Li
    Yin, Cheng-Hong
    Wu, Yu-Mei
    MEDICINE, 2017, 96 (17)
  • [3] The Impact of Excisional Treatment for Cervical Intraepithelial Neoplasia on the Vaginal Microbiota.
    Mitra, Anita
    MacIntyre, David
    Lai, Jonathan
    Lee, Yun
    Smith, Ann
    Marchesi, Julian
    Lyons, Deirdre
    Bennett, Phillip
    Kyrgiou, Maria
    REPRODUCTIVE SCIENCES, 2017, 24 : 89A - 89A
  • [4] Cervical and vaginal intraepithelial neoplasia in adolescents
    Tacla, M
    Tubaki, ME
    Schwarzschild, MMS
    Luca, PD
    Meniconi, MC
    Barrueco, AK
    Lopes, EA
    Duarte, MIS
    EUROGIN 2000: 4TH INTERNATIONAL MULTIDISCIPLINARY CONGRESS, 2000, : 291 - 295
  • [5] The vaginal microbiota associates with the regression of untreated cervical intraepithelial neoplasia 2 lesions
    Mitra, Anita
    MacIntyre, David A.
    Ntritsos, George
    Smith, Ann
    Tsilidis, Konstantinos K.
    Marchesi, Julian R.
    Bennett, Phillip R.
    Moscicki, Anna-Barbara
    Kyrgiou, Maria
    NATURE COMMUNICATIONS, 2020, 11 (01)
  • [6] The vaginal microbiota associates with the regression of untreated cervical intraepithelial neoplasia 2 lesions
    Anita Mitra
    David A. MacIntyre
    George Ntritsos
    Ann Smith
    Konstantinos K. Tsilidis
    Julian R. Marchesi
    Phillip R. Bennett
    Anna-Barbara Moscicki
    Maria Kyrgiou
    Nature Communications, 11
  • [7] Characterisation of the vaginal microbiome in cervical intraepithelial neoplasia
    Mitra, Anita
    Maclntyre, David
    Lee, Yun
    Smith, Ann
    Marchesi, Julian
    Lehne, Benjamin
    Bhatia, Ramya
    Lyons, Deirdre
    Paraskevaidis, Evangelos
    Li, Jia
    Holmes, Elaine
    Nicholson, Jeremy
    Bennett, Phillip
    Kyrgiou, Maria
    LANCET, 2016, 387 : 75 - 75
  • [8] THE VAGINAL MICROBIOME OF WOMEN WITH CERVICAL INTRAEPITHELIAL NEOPLASIA
    Mitra, A.
    MacIntyre, D. A.
    Lee, Y. S.
    Smith, A.
    Marchesi, J. R.
    Holmes, E.
    Nicholson, J. K.
    Bennett, P. R.
    Kyrgiou, M.
    INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2015, 25 (09) : 976 - 977
  • [9] Analysis of related factors of cervical intraepithelial neoplasia complicated with vaginal intraepithelial neoplasia
    Y.Y. Zhang
    R. Xia
    D. Chen
    X. Zhang
    Clinical and Translational Oncology, 2022, 24 : 902 - 908
  • [10] Recurrent vaginal intraepithelial neoplasia after hysterectomy for the treatment of cervical intraepithelial neoplasia
    Kim, J.
    Suh, D. H.
    Kim, K.
    No, J. H.
    Kim, Y. B.
    GYNECOLOGIC ONCOLOGY, 2020, 159 : 351 - 351