Electrophoretic Time-of-Flight Measurements of Single DNA Molecules with Two Stacked Nanopores

被引：50
|
作者：
Langecker, Martin [1 ,2 ,3 ,4 ]
Pedone, Daniel [3 ,4 ]
Simmel, Friedrich C. [1 ,2 ]
Rant, Ulrich [3 ,4 ]
机构：
[1] Tech Univ Munich, Lehrstuhl Bioelektron Phys Dept, D-85748 Garching, Germany
[2] Tech Univ Munich, ZNN WSI, D-85748 Garching, Germany
[3] Tech Univ Munich, Walter Schottky Inst, D-85748 Garching, Germany
[4] Tech Univ Munich, Inst Adv Study, D-85748 Garching, Germany
来源：
NANO LETTERS | 2011年 / 11卷 / 11期
关键词：
Nanopore; time-of-flight; DNA; mobility; zeta potential; translocation; SOLID-STATE NANOPORES; FORCE SPECTROSCOPY; HAIRPIN MOLECULES; TRANSLOCATION; COMPLEXES; MEMBRANES;
D O I：
10.1021/nl2030079
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Electrophoretic transport through a solid-state nanodevice comprised of two stacked nanopore sensors is used to determine the free-solution mobility of DNA molecules based on their "time-of-flight" between the two pores. Mobility measurements are possible at very low (100 pM) DNA concentration and for low as well as high salt concentrations (here 30 mM and 1 M KCl). The mechanism of DNA transport through the device is elucidated by statistical analysis, showing the free-draining nature of the translocating DNA polymers and a barrier-dominated escape through the second pore. Furthermore, consecutive threading of single molecules through the two pores can be used to gain more detailed information on the dynamics of the molecules by correlation analysis, which also provides a direct electrical proof for translocation.
引用
收藏
页码：5002 / 5007
页数：6
相关论文
共 50 条
  • [21] A TIME-OF-FLIGHT SPECTROMETER FOR MASS MEASUREMENTS AT LAMPF
    VIEIRA, DJ
    BUTLER, GW
    CLARK, JL
    WOUTERS, JM
    POSKANZER, AM
    REMSBERG, LP
    WOLLNIK, H
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1982, 183 (MAR): : 18 - NUCL
  • [22] Time-of-flight measurements in inhomogeneous electric fields
    Emelianova, E. V.
    Arkhipov, V. I.
    Adriaenssens, G. J.
    JOURNAL OF NON-CRYSTALLINE SOLIDS, 2006, 352 (9-20) : 1122 - 1125
  • [23] Neutron time-of-flight measurements of diffuse scattering
    Keen, D.A.
    Harris, M.J.
    David, W.I.F.
    Physica B: Condensed Matter, 1997, 241-243 : 201 - 203
  • [24] MAGNETIC QUADRUPOLE SPECTROMETER FOR TIME-OF-FLIGHT MEASUREMENTS
    IKEZOE, H
    TOMITA, Y
    SHIKAZONO, N
    MURAKAMI, T
    NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH, 1981, 190 (03): : 515 - 522
  • [25] INTERPRETATION OF OPTICAL TIME-OF-FLIGHT MEASUREMENTS IN SI
    TAMOR, MA
    WOLFE, JP
    PHYSICAL REVIEW B, 1988, 38 (08): : 5628 - 5633
  • [26] Time-of-flight mass measurements of exotic nuclei
    Matos, M.
    Estrade, A.
    Schatz, H.
    Bazin, D.
    Famiano, M.
    Gade, A.
    George, S.
    Lynch, W. G.
    Meisel, Z.
    Portillo, M.
    Rogers, A.
    Shapira, D.
    Stolz, A.
    Wallace, M.
    Yurkon, J.
    NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2012, 696 : 171 - 179
  • [27] On the figure of merit in neutron time-of-flight measurements
    Coceva, C
    Frisoni, M
    Magnani, M
    Mengoni, A
    NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2002, 489 (1-3): : 346 - 356
  • [28] COLD SILICON DETECTORS FOR TIME-OF-FLIGHT MEASUREMENTS
    BRUNETTI, MT
    CODINO, A
    FEDERICO, C
    GIACOMUCCI, A
    MENICHELLI, M
    MINELLI, G
    MIOZZA, M
    SALVATORI, I
    GRIMANI, C
    NUOVO CIMENTO DELLA SOCIETA ITALIANA DI FISICA A-NUCLEI PARTICLES AND FIELDS, 1993, 106 (05): : 585 - 593
  • [29] Time-of-flight measurements at the Super-FRS
    Nociforo, C.
    JOURNAL OF INSTRUMENTATION, 2014, 9
  • [30] Seeing Topological Order in Time-of-Flight Measurements
    Alba, E.
    Fernandez-Gonzalvo, X.
    Mur-Petit, J.
    Pachos, J. K.
    Garcia-Ripoll, J. J.
    PHYSICAL REVIEW LETTERS, 2011, 107 (23)
12345