An in vivo model for evaluating wound repair and regeneration microenvironments

Background: Factor differentially regulating regeneration and repair as contrasting resolutions to injury are unknown. We adapted a common wound healing model to further characterize mammalian wound repair and regeneration microenvironments. Methods: Polyvinyl alcohol sponges, sponges containing minced muscle, or blocks of minced muscle were implanted onto the backs of Fischer rats. Vascularization was assessed by infusion with india ink and progress of regeneration was evaluated histologically. Results: Regeneration occurring within sponges was histologically similar to that of free muscle blocks; but was initiated more slowly. Vascularization of minced muscle implanted in sponges was delayed slightly compared to implanted free muscle blocks. Conclusions: Regeneration of minced muscle in sponges parallels normal regeneration of free minced muscle. Incorporation into a wound repair model provides access to interstitial fluids conditioned by regenerating muscle and will lend to move detailed comparisons of the content and properties of repair and regeneration microenvironments.