Voltammetric, UV - Vis Spectrometric and Fluorescence Study of the Interaction of Ractopamine and DNA with the Aid of Multivariate Curve Resolution-Alternating Least Squares

被引：30
|
作者：
Ni, Yongnian [1 ,2 ]
Wang, Yingxia [2 ]
Kokot, Serge [3 ]
机构：
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China
[2] Nanchang Univ, Dept Chem, Nanchang 330047, Peoples R China
[3] Queensland Univ Technol, Sch Phys & Chem Sci, Brisbane, Qld 4001, Australia
来源：
ELECTROANALYSIS | 2010年 / 22卷 / 19期
基金：
中国国家自然科学基金;
关键词：
Ractopamine; DNA; Voltammetry; Fluorescence; Multivariate curve resolution-alternating least squares; UV-vis spectroscopy; NEAR-INFRARED SPECTROSCOPY; MASS-SPECTROMETRY; METAL-COMPLEXES; NUCLEIC-ACIDS; BINDING; SURFACE; DRUGS; ELECTROCHEMISTRY; CHEMOMETRICS; POLAROGRAPHY;
D O I：
10.1002/elan.200900596
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Interaction of a steroid drug, Ractopamine (RAC), and DNA was investigated by electroanalysis-differential pulse and cyclic voltammetry (DPV and CV), and UV-vis and fluorescence spectroscopy. DPV showed that RAC intercalated with DNA, and CV indicated that the reaction mechanism of RAC and dsDNA involved irreversible oxidation with the loss of two H(+) and a transfer of two electrons. Reaction binding parameters were obtained. Pure spectra of RAC, DNA and the RAC-DNA complex, and their concentrations were extracted by multivariate curve resolution-alternating least squares method (MCR-ALS). Concentration profiles indicated quantitatively the course of the reaction.
引用
收藏
页码：2216 / 2224
页数：9
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