A novel sodium bis(2-ethylhexyl) sulfosuccinate-PAGE system suitable for the separation of small peptides

被引：3
|
作者：
Lu, Xuemei [1 ]
Yao, Yao [1 ]
Zhang, Weican [1 ]
机构：
[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China
来源：
ELECTROPHORESIS | 2007年 / 28卷 / 18期
关键词：
electrophoresis; small peptide; sodium bis(2-ethylhexyl); sulfosuccinate;
D O I：
10.1002/elps.200600779
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel sodium bis(2-ethylhexyl) sulfosuccinate-PAGE (AOT-PAGE) system which delivers high resolution and sensitivity for small peptides with molecular masses of 0.8-17 kDa is described. Small peptides migrate more slowly and are less prone to leakage than in conventional SDS-PAGE, thus allowing for the in-gel detection with CBB R 250 of 0.5 mu g of peptide. The system is also compatible with electroblotting, activity staining in renatured gels, and the peptide analysis by MALDI-MS. AOT-PAGE is simpler, more rapid, and cheaper than the generally adopted Tricine-SDS-PAGE method.
引用
收藏
页码：3174 / 3177
页数：4
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