A novel sodium bis(2-ethylhexyl) sulfosuccinate-PAGE system suitable for the separation of small peptides

被引：3
|
作者：
Lu, Xuemei [1 ]
Yao, Yao [1 ]
Zhang, Weican [1 ]
机构：
[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China
来源：
ELECTROPHORESIS | 2007年 / 28卷 / 18期
关键词：
electrophoresis; small peptide; sodium bis(2-ethylhexyl); sulfosuccinate;
D O I：
10.1002/elps.200600779
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel sodium bis(2-ethylhexyl) sulfosuccinate-PAGE (AOT-PAGE) system which delivers high resolution and sensitivity for small peptides with molecular masses of 0.8-17 kDa is described. Small peptides migrate more slowly and are less prone to leakage than in conventional SDS-PAGE, thus allowing for the in-gel detection with CBB R 250 of 0.5 mu g of peptide. The system is also compatible with electroblotting, activity staining in renatured gels, and the peptide analysis by MALDI-MS. AOT-PAGE is simpler, more rapid, and cheaper than the generally adopted Tricine-SDS-PAGE method.
引用
收藏
页码：3174 / 3177
页数：4
相关论文
共 50 条
  • [1] Comparative studies of structurally similar surfactants: Sodium bis(2-ethylhexyl)phosphate and sodium bis(2-ethylhexyl)sulfosuccinate
    Luan, YX
    Xu, GY
    Yuan, SL
    Xiao, L
    Zhang, ZQ
    LANGMUIR, 2002, 18 (22) : 8700 - 8705
  • [2] Poly(vinyl pyrrolidone)-Sodium bis(2-ethylhexyl)sulfosuccinate Complex as Probe to Study the Inclusion Complex of β-Cyclodextrin with Sodium bis(2-ethylhexyl)sulfosuccinate in Aqueous Solution
    Chen, Xiaoming
    ASIAN JOURNAL OF CHEMISTRY, 2014, 26 (01) : 1 - 4
  • [3] MICROSTRUCTURES OF CONCENTRATED WATER-IN-OIL MICROEMULSIONS IN SODIUM BIS(2-ETHYLHEXYL) SULFOSUCCINATE AND SODIUM BIS(2-ETHYLHEXYL) PHOSPHATE SYSTEMS
    KURUMADA, K
    SHIOI, A
    HARADA, M
    JOURNAL OF PHYSICAL CHEMISTRY, 1994, 98 (47): : 12382 - 12389
  • [4] Electrodeposition of polypyrrole on aluminium in the presence of sodium bis(2-ethylhexyl) sulfosuccinate
    Lehr, I. L.
    Saidman, S. B.
    MATERIALS CHEMISTRY AND PHYSICS, 2006, 100 (2-3) : 262 - 267
  • [5] WATER ACTIVITY IN REVERSED SODIUM BIS(2-ETHYLHEXYL) SULFOSUCCINATE MICELLES
    POLITI, MJ
    CHAIMOVICH, H
    JOURNAL OF PHYSICAL CHEMISTRY, 1986, 90 (02): : 282 - 287
  • [6] Influence of sodium bis(2-ethylhexyl) sulfosuccinate (AOT) on zinc electrodeposition
    Lehr, I. L.
    Saidman, S. B.
    APPLIED SURFACE SCIENCE, 2012, 258 (10) : 4417 - 4423
  • [7] Flocculation of sodium bis(2-ethylhexyl)sulfosuccinate reversed micelles in isooctane
    Goto, A
    Kuwahara, Y
    Suzuki, A
    Yoshioka, H
    Goto, R
    Iwamoto, T
    Imae, T
    JOURNAL OF MOLECULAR LIQUIDS, 1997, 72 (1-3) : 137 - 144
  • [8] Phase behavior of the orange essential oil/sodium bis(2-ethylhexyl)sulfosuccinate/water system
    Feitosa, Eloi
    Cavalcante, Vanessa R. O.
    Amaral, Lia Q.
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2009, 348 (1-3) : 82 - 86
  • [9] Adsorption of sodium bis(2-ethylhexyl) sulfosuccinate and wettability in polytetrafluoroethylene-solution-air system
    Harkot, Joanna
    Janczuk, Bronislaw
    APPLIED SURFACE SCIENCE, 2007, 253 (17) : 7166 - 7171
  • [10] Spectroscopic characterization of solubilized water in reversed micelles and microemulsions:: Sodium bis(2-ethylhexyl) sulfosuccinate and sodium bis(2-ethylhexyl) phosphate in n-heptane
    Zhou, NF
    Li, Q
    Wu, JG
    Chen, J
    Weng, SF
    Xu, GX
    LANGMUIR, 2001, 17 (15) : 4505 - 4509
12345