A ligand that Trypanosoma cruzi uses to bind to mammalian cells to initiate infection

被引：28

作者：

Villalta, F ^{[1
]}

Smith, CM ^{[1
]}

Ruiz-Ruano, A ^{[1
]}

Lima, MF ^{[1
]}

机构：

[1] Meharry Med Coll, Sch Med, Dept Microbiol, Nashville, TN 37208 USA

来源：

FEBS LETTERS | 2001年 / 505卷 / 03期

关键词：

host cell receptor for Trypanosoma cruzi; gp83 trans-sialidase ligand; host cell receptor for gp83 trans-sialidase; Trypanosoma cruzi ligand; Trypanosoma cruzi attachment and entry;

D O I：

10.1016/S0014-5793(01)02853-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We purified a soluble gp83 trans-sialidase (gp83-TSA), from phospholipase C-treated Trypanosoma cruzi trypomastigote membranes, which binds to myoblasts, fibroblasts and macrophages to mediate trypanosome entry. Myoblasts display a single class of receptors for the gp83-TSA present at 4 X 10(4) per myoblast with a K-d of 8 nM. Monovalent Fab fragments of the monoclonal antibody 4A4 specific for gp83-TSA inhibit gp83-TSA binding to myoblasts, fibroblasts and macrophages, block the trypanosomes from attaching to and entering these cells and neutralize T. cruzi infection in BALB/c mice. This is the first demonstration that gp83-TSA is a ligand that T. cruzi uses to attach to cells. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：383 / 388

页数：6

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