A ligand that Trypanosoma cruzi uses to bind to mammalian cells to initiate infection

被引：28
|
作者：
Villalta, F [1 ]
Smith, CM [1 ]
Ruiz-Ruano, A [1 ]
Lima, MF [1 ]
机构：
[1] Meharry Med Coll, Sch Med, Dept Microbiol, Nashville, TN 37208 USA
来源：
FEBS LETTERS | 2001年 / 505卷 / 03期
关键词：
host cell receptor for Trypanosoma cruzi; gp83 trans-sialidase ligand; host cell receptor for gp83 trans-sialidase; Trypanosoma cruzi ligand; Trypanosoma cruzi attachment and entry;
D O I：
10.1016/S0014-5793(01)02853-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We purified a soluble gp83 trans-sialidase (gp83-TSA), from phospholipase C-treated Trypanosoma cruzi trypomastigote membranes, which binds to myoblasts, fibroblasts and macrophages to mediate trypanosome entry. Myoblasts display a single class of receptors for the gp83-TSA present at 4 X 10(4) per myoblast with a K-d of 8 nM. Monovalent Fab fragments of the monoclonal antibody 4A4 specific for gp83-TSA inhibit gp83-TSA binding to myoblasts, fibroblasts and macrophages, block the trypanosomes from attaching to and entering these cells and neutralize T. cruzi infection in BALB/c mice. This is the first demonstration that gp83-TSA is a ligand that T. cruzi uses to attach to cells. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：383 / 388
页数：6
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