The seed fatty acids composition from five Spanish pomegranate cultivars was determined. Pomegranate seeds yielded oil contents ranging from 69-81 g kg(-1) of dry matter for sweet cv. to 131 g kg(-1) and 105 g kg(-1) for the sour-sweet and sour cv., respectively. The highest levels of fatty acids were those of polyunsaturated ones (from 80.41 to 91.03%). The predominant unsaturated fatty acid was punicic (66.76-79.29%). Data on punicic acids were reported here for the first time for sour and sour-sweet Spanish pomegranate cv. This relatively high quantity of unsaturated fatty acids could be of interest for human nutrition and may definitively drive breeding selection programs.