A predicted ABC transporter, FtsEX, is needed for cell division in Escherichia coli

被引:150
|
作者
Schmidt, KL
Peterson, ND
Kustusch, RJ
Wissel, MC
Graham, B
Phillips, GJ
Weiss, DS [1 ]
机构
[1] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA
[2] Iowa State Univ, Vet Med Res Inst, Dept Vet Microbiol, Ames, IA 50011 USA
关键词
D O I
10.1128/JB.186.3.785-793.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
FtsE and FtsX have homology to the ABC transporter superfamily of proteins and appear to be widely conserved among bacteria. Early work implicated FtsEX in cell division in Escherichia coli, but this was subsequently challenged, in part because the division defects in ftsEX mutants are often salt remedial. Strain RG60 has an ftsE::kan null mutation that is polar onto ftsX. RG60 is mildly filamentous when grown in standard Luria-Bertani medium (LB), which contains 1% NaCl, but upon shift to LB with no NaCl growth and division stop. We found that FtsN localizes to potential division sites, albeit poorly, in RG60 grown in LB with 1% NaCl. We also found that in wild-type E. coli both FtsE and FtsX localize to the division site. Localization of FtsX was studied in detail and appeared to require FtsZ, FtsA, and ZipA, but not the downstream division proteins Fts K, FtsQ, FtsL, and FtsI. Consistent with this, in media lacking salt, FtsA and ZipA localized independently of FtsEX, but the downstream proteins did not. Finally, in the absence of salt, cells depleted of FtsEX stopped dividing before any change in growth rate (mass increase) was apparent. We conclude that FtsEX participates directly in the process of cell division and is important for assembly or stability of the septal ring, especially in salt-free media.
引用
收藏
页码:785 / 793
页数:9
相关论文
共 50 条
  • [21] A synthetic system for asymmetric cell division in Escherichia coli
    Molinari, Sara
    Shis, David L.
    Bhakta, Shyam P.
    Chappell, James
    Igoshin, Oleg A.
    Bennett, Matthew R.
    NATURE CHEMICAL BIOLOGY, 2019, 15 (09) : 917 - +
  • [22] CELL-DIVISION ENZYMES IN ESCHERICHIA-COLI
    ISHINO, F
    NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, 1989, 63 (11): : 1755 - 1764
  • [23] REGULATION OF CELL-DIVISION IN ESCHERICHIA-COLI
    LUTKENHAUS, J
    TRENDS IN GENETICS, 1990, 6 (01) : 22 - 25
  • [24] Effects of chromosome underreplication on cell division in Escherichia coli
    Botello, E
    Nordström, K
    JOURNAL OF BACTERIOLOGY, 1998, 180 (23) : 6364 - 6374
  • [25] A synthetic system for asymmetric cell division in Escherichia coli
    Sara Molinari
    David L. Shis
    Shyam P. Bhakta
    James Chappell
    Oleg A. Igoshin
    Matthew R. Bennett
    Nature Chemical Biology, 2019, 15 : 917 - 924
  • [26] The Interplay of ClpXP with the Cell Division Machinery in Escherichia coli
    Camberg, Jodi L.
    Hoskins, Joel R.
    Wickner, Sue
    JOURNAL OF BACTERIOLOGY, 2011, 193 (08) : 1911 - 1918
  • [27] CELL DIVISION OF ESCHERICHIA-COLI-LON MUTANT
    WALKER, JR
    SMITH, JA
    MOLECULAR AND GENERAL GENETICS, 1970, 108 (03): : 249 - +
  • [28] MALTOSE TRANSPORT-SYSTEM OF ESCHERICHIA-COLI - AN ABC-TYPE TRANSPORTER
    NIKAIDO, H
    FEBS LETTERS, 1994, 346 (01) : 55 - 58
  • [29] Function of Escherichia coli MsbA, an essential ABC family transporter, in lipid A and phospholipid biosynthesis
    Zhou, ZM
    White, KA
    Polissi, A
    Georgopoulos, C
    Raetz, CRH
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (20) : 12466 - 12475
  • [30] Cloning and Functional Characterization of Putative Escherichia coli ABC Multidrug Efflux Transporter YddA
    Feng, Zhenyue
    Liu, Defu
    Liu, Ziwen
    Liang, Yimin
    Wang, Yanhong
    Liu, Qingpeng
    Liu, Zhenhua
    Zang, Zhongjing
    Cui, Yudong
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 30 (07) : 982 - 995