Advances in Native Chemical Ligation-Desulfurization: A Powerful Strategy for Peptide and Protein Synthesis

被引：44

作者：

Jin, Kang ^{[1
]}

Li, Xuechen ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Chem, State Key Lab Synthet Chem, Hong Kong, Hong Kong, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2018年 / 24卷 / 66期

基金：

中国国家自然科学基金;

关键词：

cysteine surrogates; desulfurization; native chemical ligation (NCL); one-pot synthesis; proteins; SUGAR-ASSISTED LIGATION; HYPOPHOSPHITE COMBINATION SYSTEM; REDUCTIVE DESULFURIZATION; VISIBLE-LIGHT; CYSTEINE; GLYCOPEPTIDE; NICKEL; ACID; POLYPEPTIDES; RACEMIZATION;

D O I：

10.1002/chem.201802067

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cysteine-based native chemical ligation (NCL) has been a very powerful approach for convergent synthesis of peptides and proteins. However, owing to the low abundance of cysteine (Cys) in proteins, applications of NCL in protein chemical synthesis are limited. To expand the peptide ligation toolbox, NCL followed by desulfurization has been developed to enable peptide ligation at Xaa-Ala conjunctions, that is, formal "alanine ligation". In this regard, effective peptide desulfurization methods are critical. This Concept article summarizes the development of different desulfurization strategies for peptide and protein chemical synthesis.

引用

页码：17397 / 17404

页数：8

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