Determination of five antiarrhythmic drugs in human plasma by dispersive liquid-liquid microextraction and high-performance liquid chromatography

被引:61
|
作者
Jouyban, Abolghasem [1 ,2 ]
Sorouraddin, Mohammad Hossein [3 ]
Farajzadeh, Mir Ali [3 ]
Somi, Mohammad Hossein [4 ]
Fazeli-Bakhtiyari, Rana [3 ,4 ]
机构
[1] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran
[2] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran
[3] Univ Tabriz, Fac Chem, Dept Analyt Chem, Tabriz, Iran
[4] Tabriz Univ Med Sci, Liver & Gastrointestinal Dis Res Ctr, Tabriz, Iran
关键词
Dispersive liquid-liquid microextraction; High-performance liquid chromatography-ultraviolet; Method validation; Antiarrhythmic drugs; Plasma; SOLID-PHASE EXTRACTION; TANDEM MASS-SPECTROMETRY; CAPILLARY-ZONE-ELECTROPHORESIS; FLOATING ORGANIC DROPLET; BETA-BLOCKER DRUGS; HUMAN URINE; CARDIOVASCULAR DRUGS; GAS-CHROMATOGRAPHY; SCREENING METHOD; DOPING AGENTS;
D O I
10.1016/j.talanta.2014.12.008
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A fast and sensitive high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection was developed and validated for the simultaneous quantitation of five antiarrhythmic drugs (metoprolol, propranolol, carvedilol, diltiazem, and verapamil) in human plasma samples. It involves dispersive liquid-liquid microextraction (DLLME) of the desired drugs from 660 mu L plasma and separation using isocratic elution with UV detection at 200 nm. The complete separation of all analytes was achieved within 7 min. Acetonitrile (as disperser solvent) resulting from the protein precipitation procedure was mixed with 100 mu L dichloromethane (as an extraction solvent) and rapidly injected into 5 mL aqueous solution (pH 11.5) containing 1% (w/v), NaCl. After centrifugation, the sedimented phase containing enriched analytes was collected and evaporated to dryness. The residue was re-dissolved in 50 mu L deionized water (acidified to pH 3) and injected into the HPLC system for analysis. Under the optimal conditions, the enrichment factors and extraction recoveries ranged between 4.4-10.8 and 33-82%, respectively. The suggested method was linear (r(2) >= 0.997) over a dynamic range of 0.02-0.80 mu g mL(-1) in plasma. The intra- and inter-days relative standard deviation (RSD%) and relative error (RE%) values of the method were below 20%, which shows good precision and accuracy. Finally, this method was applied to the analysis of real plasma samples obtained from the patients treated with these drugs. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:681 / 689
页数:9
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