A fast and sensitive high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection was developed and validated for the simultaneous quantitation of five antiarrhythmic drugs (metoprolol, propranolol, carvedilol, diltiazem, and verapamil) in human plasma samples. It involves dispersive liquid-liquid microextraction (DLLME) of the desired drugs from 660 mu L plasma and separation using isocratic elution with UV detection at 200 nm. The complete separation of all analytes was achieved within 7 min. Acetonitrile (as disperser solvent) resulting from the protein precipitation procedure was mixed with 100 mu L dichloromethane (as an extraction solvent) and rapidly injected into 5 mL aqueous solution (pH 11.5) containing 1% (w/v), NaCl. After centrifugation, the sedimented phase containing enriched analytes was collected and evaporated to dryness. The residue was re-dissolved in 50 mu L deionized water (acidified to pH 3) and injected into the HPLC system for analysis. Under the optimal conditions, the enrichment factors and extraction recoveries ranged between 4.4-10.8 and 33-82%, respectively. The suggested method was linear (r(2) >= 0.997) over a dynamic range of 0.02-0.80 mu g mL(-1) in plasma. The intra- and inter-days relative standard deviation (RSD%) and relative error (RE%) values of the method were below 20%, which shows good precision and accuracy. Finally, this method was applied to the analysis of real plasma samples obtained from the patients treated with these drugs. (C) 2014 Elsevier B.V. All rights reserved.
机构:
Univ Fed Pernambuco, Dept Pharmaceut Sci, Prof Artur de Sa S-N,Cidade Univ, BR-50740521 Recife, PE, BrazilUniv Fed Pernambuco, Dept Pharmaceut Sci, Prof Artur de Sa S-N,Cidade Univ, BR-50740521 Recife, PE, Brazil
da Silva, Pedro Rafael
Santana Sena, Lais Cristina
论文数: 0引用数: 0
h-index: 0
机构:
Secretary Social Def Pernambuco, Criminalist Inst, Lab Forens Toxicol, Marques de Pombal 455, BR-50100170 Recife, PE, BrazilUniv Fed Pernambuco, Dept Pharmaceut Sci, Prof Artur de Sa S-N,Cidade Univ, BR-50740521 Recife, PE, Brazil
Santana Sena, Lais Cristina
Lorena Silva, Rubens Pedro
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Pernambuco, Dept Pharmaceut Sci, Prof Artur de Sa S-N,Cidade Univ, BR-50740521 Recife, PE, BrazilUniv Fed Pernambuco, Dept Pharmaceut Sci, Prof Artur de Sa S-N,Cidade Univ, BR-50740521 Recife, PE, Brazil
Lorena Silva, Rubens Pedro
Almeida Silva de Santana, Danielle Cristine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Pernambuco, Dept Pharmaceut Sci, Prof Artur de Sa S-N,Cidade Univ, BR-50740521 Recife, PE, BrazilUniv Fed Pernambuco, Dept Pharmaceut Sci, Prof Artur de Sa S-N,Cidade Univ, BR-50740521 Recife, PE, Brazil
Almeida Silva de Santana, Danielle Cristine
Malagueno de Santana, Fernando Jose
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Pernambuco, Dept Pharmaceut Sci, Prof Artur de Sa S-N,Cidade Univ, BR-50740521 Recife, PE, BrazilUniv Fed Pernambuco, Dept Pharmaceut Sci, Prof Artur de Sa S-N,Cidade Univ, BR-50740521 Recife, PE, Brazil
机构:
Kermanshah Univ Med Sci, Iran West Ctr Drug Abuse Studies, Kermanshah, IranKermanshah Univ Med Sci, Iran West Ctr Drug Abuse Studies, Kermanshah, Iran
Ahmadi-Jouibari, Toraj
Fattahi, Nazir
论文数: 0引用数: 0
h-index: 0
机构:
Kermanshah Univ Med Sci, Dept Environm Hlth Engn, Environm Epidemiol Res Ctr, Kermanshah, IranKermanshah Univ Med Sci, Iran West Ctr Drug Abuse Studies, Kermanshah, Iran
Fattahi, Nazir
Shamsipur, Mojtaba
论文数: 0引用数: 0
h-index: 0
机构:
Razi Univ, Dept Chem, Kermanshah, IranKermanshah Univ Med Sci, Iran West Ctr Drug Abuse Studies, Kermanshah, Iran
Shamsipur, Mojtaba
Pirsaheb, Meghdad
论文数: 0引用数: 0
h-index: 0
机构:
Kermanshah Univ Med Sci, Dept Environm Hlth Engn, Environm Epidemiol Res Ctr, Kermanshah, IranKermanshah Univ Med Sci, Iran West Ctr Drug Abuse Studies, Kermanshah, Iran