Engineered Combined-Positive-Control Template for Real-Time Reverse Transcription-PCR in Multiple-Pathogen-Detection Assays

被引：39
|
作者：
Kodani, Maja [1 ]
Winchell, Jonas M. [1 ]
机构：
[1] Ctr Dis Control & Prevent, Div Bacterial Dis, Resp Dis Branch, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 03期
关键词：
RESPIRATORY-TRACT; INFECTION; BACTERIAL; VIRUSES;
D O I：
10.1128/JCM.05987-11
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Recently we evaluated a custom TaqMan array card (TAC) detection system, formerly known as a TaqMan low-density array (TLDA) card, for simultaneous real-time PCR identification of 21 pathogens and three control targets in duplicate from respiratory specimens (M. Kodani et al., J. Clin. Microbiol. 49: 2175-2182, 2011). We engineered an adaptable and expandable system of in vitro RNA transcripts to serve as a combined positive control for both DNA and RNA targets in multiple-pathogen-detection technologies based on real-time reverse transcription-PCR.
引用
收藏
页码：1057 / 1060
页数：4
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