The CovS/CovR Acid Response Regulator Is Required for Intracellular Survival of Group B Streptococcus in Macrophages

被引:57
|
作者
Cumley, Nicola J. [1 ]
Smith, Leanne M. [1 ]
Anthony, Mark [2 ]
May, Robin C. [1 ]
机构
[1] Univ Birmingham, Sch Biosci, Coll Life & Environm Sci, Birmingham, AL USA
[2] John Radcliffe Hosp, Dept Neonatol, Oxford OX3 9DU, England
关键词
CRYPTOCOCCUS-NEOFORMANS; AGALACTIAE STRAINS; GENOME SEQUENCE; VIRULENCE; MECHANISM; PATHOGEN; IRON; EXPRESSION; IDENTIFICATION; CHLOROQUINE;
D O I
10.1128/IAI.05443-11
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Group B Streptococcus (GBS) is a leading cause of neonatal meningitis and septicemia. The ability of this organism to survive inside phagocytic cells is poorly understood but thought to be an important step for the establishment of disease in the host. Here, we demonstrate that GBS shows prolonged survival within J774 macrophages and that the capacity to survive is not significantly changed across a diverse range of strains representing different serotypes, multilocus sequence types (MLST), and sites of clinical isolation. Using staining for the lysosome-associated membrane protein (LAMP) and by pharmacological inhibition of phagosome acidification, we demonstrate that streptococci reside in a phagosome and that acidification of the phagosome is required for GBS to survive intracellularly. Moreover, we show that the GBS two-component system CovS/CovR, which is the major acid response regulator in this organism, is required for survival inside the phagosome.
引用
收藏
页码:1650 / 1661
页数:12
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