PTZ-induced seizures inhibit adenosine deamination in adult zebrafish brain membranes

被引:27
|
作者
Siebel, Anna Maria [1 ,2 ]
Piato, Angelo Luis [1 ,2 ,3 ]
Capiotti, Katiucia Marques [1 ,2 ]
Seibt, Kelly Juliana [1 ,2 ]
Bogo, Mauricio Reis [2 ,4 ]
Bonan, Carla Denise [1 ,2 ]
机构
[1] Pontificia Univ Catolica Rio Grande do Sul, Lab Neuroquim & Psicofarmacol, Programa Posgrad Biol Celular & Mol, Dept Biol Celular & Mol,Fac Biociencias, BR-90619900 Porto Alegre, RS, Brazil
[2] INCT TM, BR-90035003 Porto Alegre, RS, Brazil
[3] Pontificia Univ Catolica Rio Grande do Sul, Programa Posgrad Med & Ciencias Saude, BR-90610000 Porto Alegre, RS, Brazil
[4] Pontificia Univ Catolica Rio Grande do Sul, Lab Biol Celular & Mol, Programa Posgrad Biol Celular & Mol, Dept Biol Celular & Mol,Fac Biociencias, BR-90619900 Porto Alegre, RS, Brazil
关键词
Adenosine; Adenosine deaminase; Ectonucleotidases; Seizures; Pentylenetetrazole; Zebrafish; NERVOUS-SYSTEM; EPILEPSY; PROTEIN; RECEPTORS; ATP; HYDROLYSIS; MECHANISMS; EXPRESSION; INSIGHTS; BW534U87;
D O I
10.1016/j.brainresbull.2011.08.017
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Adenosine exerts neuromodulatory functions with mostly inhibitory effects, being considered an endogenous anticonvulsant. The hydrolysis of ATP by ectonucleotidases is an important source of adenosine, and adenosine deaminase (ADA) contributes to the regulation of this nucleoside concentration through its deamination. In this study, we tested the effect of pentylenetetrazole (PTZ)-induced seizures on ectonucleotidase and ADA activities in adult zebrafish brain. Our results have demonstrated that PTZ treatments did not alter ectonucleotidase and ADA activities in membranes and soluble fraction, respectively. However, ecto-ADA activity was significantly decreased in brain membranes of animals exposed to 5 mM and 15 mM PTZ treatments (22.4% and 29.5%, respectively) when compared to the control group. Semiquantitative RT-PCR analysis did not show significant changes after the PTZ exposure on ADA gene expression. The decreased adenosine deamination observed in this study suggests a modulation of extracellular adenosine levels during PTZ-induced seizures in zebrafish. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:385 / 389
页数:5
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