Quantitative analysis of platelets aggregates in 3D by digital holographic microscopy

被引：15

作者：

Boudejltia, Karim Zouaoui ^{[1
]}

de Sousa, Daniel Ribeiro ^{[1
]}

Uzureau, Pierrick ^{[1
]}

Yourassowsky, Catherine ^{[2
]}

Perez-Morga, David ^{[3
]}

Courbebaisse, Guy ^{[4
]}

Chopard, Bastien ^{[5
]}

Dubois, Frank ^{[2
]}

机构：

[1] Univ Libre Bruxelles, CHU Charleroi, Fac Med, Lab Med Expt,ULB Unit 222, Brussels, Belgium

[2] Univ Libre Bruxelles, Micrograv Res Ctr, Brussels, Belgium

[3] Univ Libre Bruxelles, IBMM, Lab Mol Parasitol, Gosselies, Belgium

[4] Univ Lyon, INSA Lyon, UCB Lyon1, CREATIS,CNRS,UMR 5220,INSERM,U1044, Lyon, France

[5] Univ Geneva, Dept Informat, Geneva, Switzerland

来源：

BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 09期

关键词：

ACTIVATION; MECHANISMS; ADHESION; DYNAMICS;

D O I：

10.1364/BOE.6.003556

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Platelet spreading and retraction play a pivotal role in the platelet plugging and the thrombus formation. In routine laboratory, platelet function tests include exhaustive information about the role of the different receptors present at the platelet surface without information on the 3D structure of platelet aggregates. In this work, we develop, a method in Digital Holographic Microscopy (DHM) to characterize the platelet and aggregate 3D shapes using the quantitative phase contrast imaging. This novel method is suited to the study of platelets physiology in clinical practice as well as the development of new drugs. (C)2015 Optical Society of America

引用

页码：3556 / 3563

页数：8

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