Holographic 3D fluorescence microscopy

被引：0

作者：

Clark, David C. ^{[1
]}

Jang, Changwon ^{[2
]}

Kim, Jonghyun ^{[2
]}

Lee, Byoungho ^{[2
]}

Kim, Myung K. ^{[1
]}

机构：

[1] Univ S Florida, Dept Phys, Tampa, FL 33620 USA

[2] Seoul Natl Univ, Sch Elect Engn, Seoul, South Korea

来源：

FIFTH ASIA-PACIFIC OPTICAL SENSORS CONFERENCE | 2015年 / 9655卷

关键词：

SIDH; differential imaging; fluorescence microscopy; incoherent holography; three-dimensional tracking; aberration; adaptive optics;

D O I：

10.1117/12.2185087

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Fluorescence microscopy is an indispensable imaging tool in modern biomedical research. Holography is well-known to have many interesting and useful imaging capabilities. But the requirement of coherent illumination has all but precluded holography as a means for fluorescence imaging, which is inherently incoherent. Recent developments in digital holography, however, including self-interference incoherent digital holography (SIDH), provide highly effective and versatile capabilities for 3D holographic imaging with incoherent light, that can remove the barrier between fluorescence and holography. Recent progress in holographic fluorescence microscopy is presented.

引用

页数：4

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