Imaging of surface microdomains on individual extracellular vesicles in 3-D

被引：39

作者：

McNamara, Ryan P. ^{[1
,2
]}

Zhou, Yijun ^{[1
,2
]}

Eason, Anthony B. ^{[1
,2
]}

Landis, Justin T. ^{[1
,2
]}

Chambers, Meredith G. ^{[1
,2
]}

Willcox, Smaranda ^{[2
]}

Peterson, Tiffany A. ^{[3
]}

Schouest, Blake ^{[3
,5
]}

Maness, Nicholas J. ^{[3
]}

MacLean, Andrew G. ^{[3
]}

Costantini, Lindsey M. ^{[4
]}

Griffith, Jack D. ^{[2
]}

Dittmer, Dirk Peter ^{[1
,2
]}

机构：

[1] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC USA

[3] Tulane Univ, Tulane Natl Primate Res Ctr, Covington, LA USA

[4] North Carolina Cent Univ, Dept Biol & Biomed Sci, Durham, NC USA

[5] La Jolla Inst Immunol, Ctr Infect Dis & Vaccine Res, La Jolla, CA USA

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2022年 / 11卷 / 03期

基金：

美国国家卫生研究院;

关键词：

biotechnology; exosome; extracellular vesicle; microvesicle; dSTORM; nanodomains; super-resolution microscopy; tetraspanin; MESSENGER-RNAS; EXOSOMES; REVEALS; MICROSCOPY; SECRETION; BIOGENESIS; INFECTION;

D O I：

10.1002/jev2.12191

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Extracellular vesicles (EVs) are secreted from all cell types and are intimately involved in tissue homeostasis. They are being explored as vaccine and gene therapy platforms, as well as potential biomarkers. As their size is below the diffraction limit of light microscopy, direct visualizations have been daunting and single-particle studies under physiological conditions have been hampered. Here, direct stochastic optical reconstruction microscopy (dSTORM) was employed to visualize EVs in three-dimensions and to localize molecule clusters such as the tetraspanins CD81 and CD9 on the surface of individual EVs. These studies demonstrate the existence of membrane microdomains on EVs. These were confirmed by Cryo-EM. Individual particle visualization provided insights into the heterogeneity, structure, and complexity of EVs not previously appreciated

引用

页数：18

共 50 条

[41] 3-D IMAGING IN GASTROINTESTINAL LAPAROSCOPY
BIRKETT, DH
SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES, 1993, 7 (06): : 556 - 557
[42] 3-D vascular ultrasound imaging
Fenster, A
Lee, D
Downey, D
WHERE INSTRUMENTATION IS GOING - CONFERENCE PROCEEDINGS, VOLS 1 AND 2, 1998, : 559 - 561
[43] 3-D ultrasound imaging: A review
Fenster, A
Downey, DB
IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 1996, 15 (06): : 41 - 51
[44] 3-D Interferometric optoacoustic imaging
Carp, SA
Venugopalan, V
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2005, 2005, 5697 : 307 - 312
[45] 3-D Confocal Raman Imaging
Fischer, Harald
SPECTROSCOPY, 2010, : 24 - 24
[46] 3-D imaging and stereotactic radiosurgery
Yan, YL
Bao, XD
Bai, Y
Luo, LM
Shu, HZ
Fu, Y
IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 1997, 16 (04): : 47 - 52
[47] CONFOCAL MICROSCOPES FOR 3-D IMAGING
DIXON, A
BENHAM, GS
PHOTONICS SPECTRA, 1987, 21 (10) : 145 - &
[48] Easy, accurate 3-D imaging
不详
INTECH, 1998, 45 (08) : 34 - 34
[49] Confocal imaging, visualization and 3-D surface measurement of small mammalian teeth
Evans, AR
Harper, IS
Sanson, GD
JOURNAL OF MICROSCOPY, 2001, 204 : 108 - 118
[50] 3-D imaging of surface topography of wear particles found in synovial joints
Podsiadlo, P
Stachowiak, GW
WEAR, 1999, 230 (02) : 184 - 193

← 1 2 3 4 5 →