Targeting Intracellular Pathogenic Bacteria Through N-Terminal Modification of Cationic Amphiphilic Polyproline Helices

被引：9

作者：

Dietsche, Thomas A. ^{[1
]}

Eldesouky, Hassan E. ^{[2
]}

Zeiders, Samantha M. ^{[1
]}

Seleem, Mohamed N. ^{[2
]}

Chmielewski, Jean ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Comparat Pathobiol, W Lafayette, IN 47907 USA

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2020年 / 85卷 / 11期

基金：

美国国家科学基金会;

关键词：

ACINETOBACTER-BAUMANNII; NANOPARTICLES; PEPTIDES;

D O I：

10.1021/acs.joc.0c00871

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Intracellular pathogens can thrive within mammalian cells and are inaccessible to many antimicrobial agents. Herein, we present a facile method of enhancing the cell penetrating and antibacterial properties of cationic amphiphilic polyproline helices (CAPHs) with modifications to the hydrophobic moiety at the N-terminus. These altered CAPHs display superior cell penetration within macrophage cells, and in some cases, minimal cytotoxicity. Furthermore, one CAPH, Pentyl-P14 exhibited excellent antibacterial activity against multiple strains of pathogenic bacteria and promoted the clearance of intracellular Shigella within macrophages.

引用

页码：7468 / 7475

页数：8

共 42 条

[21] PREVENTING FORMATION OF TOXIC N-TERMINAL HUNTINGTIN FRAGMENTS THROUGH ANTISENSE OLIGONUCLEOTIDE-MEDIATED PROTEIN MODIFICATION
Evers, M.
Casaca-Carreira, J.
Toonen, L.
Jahanshahi, A.
Temel, Y.
van Roon-Mom, W.
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 2014, 85 : A95 - A95
[22] Preventing Formation of Toxic N-Terminal Huntingtin Fragments Through Antisense Oligonucleotide-Mediated Protein Modification
Evers, Melvin M.
Hoang-Dai Tran
Zalachoras, Ioannis
Meijer, Onno C.
den Dunnen, Johan T.
van Ommen, Gert-Jan B.
Aartsma-Rus, Annemieke
van Roon-Mom, Willeke M. C.
NUCLEIC ACID THERAPEUTICS, 2014, 24 (01) : 4 - 12
[23] Site-Specific Modification of Proteins through N-Terminal Azide Labeling and a Chelation-Assisted CuAAC Reaction
Inoue, Nozomu
Onoda, Akira
Hayashi, Takashi
BIOCONJUGATE CHEMISTRY, 2019, 30 (09) : 2427 - 2434
[24] Facile synthesis of SAM-peptide conjugates through alkyl linkers targeting protein N-terminal methyltransferase 1
Zhang, Gang
Huang, Rong
RSC ADVANCES, 2016, 6 (08): : 6768 - 6771
[25] STATISTICAL AND FUNCTIONAL ANALYSES OF VIRAL AND CELLULAR PROTEINS WITH N-TERMINAL AMPHIPATHIC ALPHA-HELICES WITH LARGE HYDROPHOBIC MOMENTS - IMPORTANCE TO MACROMOLECULAR RECOGNITION AND ORGANELLE TARGETING
SAIER, MH
MCCALDON, P
JOURNAL OF BACTERIOLOGY, 1988, 170 (05) : 2296 - 2300
[26] N-terminal alternately spliced regions of PDE4A cAMP-specific phosphodiesterases determine intracellular targeting and regulation of catalytic activity
Houslay, MD
BIOCHEMICAL SOCIETY TRANSACTIONS, 1996, 24 (04) : 980 - 986
[27] The Scavenger Receptor SSc5D Physically Interacts with Bacteria through the SRCR-Containing N-Terminal Domain
Bessa Pereira, Catarina
Bockova, Marketa
Santos, Rita F.
Santos, Ana Mafalda
de Araujo, Mafalda Martins
Oliveira, Liliana
Homola, Jiri
Carmo, Alexandre M.
FRONTIERS IN IMMUNOLOGY, 2016, 7
[28] Commentary: The Scavenger Receptor SSc5D Physically Interacts with Bacteria through the SRCR-Containing N-Terminal Domain
Lozano, Francisco
Martinez-Florensa, Mario
FRONTIERS IN IMMUNOLOGY, 2017, 8
[29] Targeting of PDE4A camp phosphodiesterases through N-terminal splice regions: Interactions with SH3 domains.
Houslay, MD
IMMUNOLOGY, 1996, 89 : SF116 - SF116
[30] Response: Commentory: The Scavenger Receptor SSc5D Physically Interacts with Bacteria through the SRCR-Containing N-Terminal Domain
Oliveira, Liliana
Carmo, Alexandre M.
FRONTIERS IN IMMUNOLOGY, 2017, 8

← 1 2 3 4 5 →