Sequencing, cloning and expression of CP4EPSPS roundup ready soybean insert

被引：0
|
作者：
Son, DY [1 ]
Ahn, KM [1 ]
Lee, SI [1 ]
机构：
[1] Sungkyunkwan Univ, Sch Med, Dept Pediat Samsung Med Ctr, Seoul 135230, South Korea
来源：
FOOD SCIENCE AND BIOTECHNOLOGY | 2003年 / 12卷 / 02期
关键词：
genetically modified food; glyphosate-tolerant soybean; 5-enol-pyruvyl-shikimate-3-phosphate synthase;
D O I：
暂无
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Results of the CP4EPSPS gene sequence in the transgenic Roundup Ready soybean, and cloning and expression of CP4EPSPS protein using bacterial expression system are described. Our sequencing results show that DNA rearrangement occurred between 3' NOS terminator and the genomic plant DNA. Expressed CP4EPSPS protein was isolated using simple two steps of DEAE column, and can be used as a source material for the allergic risk assessment of newly introduced foreign protein in soybean and for the production of mono- and/or poly-clonal antibodies.
引用
收藏
页码：133 / 136
页数：4
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