Determination of metallothionein in Daphnia magna by modified square wave cathodic stripping voltammetry

被引：6
|
作者：
Fan, Wenhong [1 ]
Wang, Xiangrui [1 ]
Li, Xiaomin [1 ]
Xue, Feng [1 ]
机构：
[1] Beihang Univ, Sch Chem & Environm, Beijing 100191, Peoples R China
来源：
ELECTROCHEMISTRY COMMUNICATIONS | 2015年 / 52卷
基金：
中国国家自然科学基金;
关键词：
Metallothionein; Square wave cathodic stripping voltammetry; Accurate determination; Inorganic anions; BIOMARKER RESPONSES; METAL ACCUMULATION; BRDICKA REACTION; CHRONOPOTENTIOMETRY; BIOACCUMULATION; OPTIMIZATION; ELECTRODES; PROTEINS; CADMIUM; TUMORS;
D O I：
10.1016/j.elecom.2015.01.008
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Metallothioneins (MTs) are widely used as biomarkers in environmental studies. However, eliminating interfering factors for MT determination in vivo by electrochemical detection is difficult. In this study, NO3- and Cl- were found to negatively affect MT determination in Daphnia magna by square wave cathodic stripping voltammetry (SWCSV). The relevant mechanism was analyzed, and results were used to modify the original electrochemical testing parameters and environmental conditions. This modified SWCSV achieved accurate MT concentrations in D. magna, leading to detection limits as low as picomolar levels. (C) 2015 Elsevier B.V. All rights reserved.
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页码：17 / 20
页数：4
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