Controlling the glycosylation of recombinant proteins expressed in animal cells by genetic and physiological engineering

被引：0
|
作者：
Lamotte, D
EonDuval, A
Acerbis, G
Distefano, G
Monaco, L
Soria, M
Jenkins, N
Engasser, JM
Marc, A
机构：
来源：
ANIMAL CELL TECHNOLOGY: FROM VACCINES TO GENETIC MEDICINE | 1997年
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D O I：
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中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The cDNA coding for rat alpha-2,6-sialyltransferase (alpha 2,6-ST) was expressed in a recombinant CHO cell line producing interferon-gamma, using a novel in vitro amplification vector. The alpha 2,6-ST was expressed efficiently, 20-30% of the sialic acid pool on interferon-gamma being linked in the alpha 2,6-conformation. Despite the overall level of sialylation was not significantly increased, growth and interferon-gamma production in bioreactor were not compromised by the transfection and expression of the gene encoding for the alpha 2,6-ST.
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页码：761 / 765
页数：5
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