Improved conditions for the removal of 2-oxoacyl groups from the N-terminus of proteins

被引：4
|
作者：
Sunde, M [1 ]
Sparkes, MJ [1 ]
Dixon, HBF [1 ]
机构：
[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1388卷 / 01期
关键词：
transamination; scission; N-terminal residue;
D O I：
10.1016/S0167-4838(98)00162-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Proteins with R-CO-CO-NH- at the N-terminus, rather than the usual R-CH(-NH3+)-CO-NH-, are produced by nonenzymic transamination and also occur in the pyruvoyl enzymes. The oxoacyl group may be specifically removed from a model peptide, in yields of 70-80%, by treating them in 0.1 M phosphate buffer at 37 degrees C for 24 h with 25 mM of the N-phosphonoaethyl derivative of phenylene-1,2-diamine. This provides mild conditions for the stepwise removal of N-terminal residues without denaturation. (C) 1998 Elsevier Science B.V. All rights reserved.
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页码：45 / 52
页数：8
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