Effect of common bean seed exudates on growth, lipopolysaccharide production, and lipopolysaccharide transport gene expression of Rhizobium anhuiense

被引:4
|
作者
Xiang, Quanju [1 ]
Wang, Jie [1 ]
Qin, Peng [1 ]
Adil, Bilal [1 ]
Xu, Kaiwei [1 ]
Gu, Yunfu [1 ]
Yu, Xiumei [1 ]
Zhao, Ke [1 ]
Zhang, Xiaoping [1 ]
Ma, Menggen [1 ]
Chen, Qiang [1 ]
Chen, Xiaoqiong [2 ]
Yan, Yanhong [3 ]
机构
[1] Sichuan Agr Univ, Coll Resource, Chengdu 611130, Sichuan, Peoples R China
[2] Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Sichuan, Peoples R China
[3] Sichuan Agr Univ, Coll Anim Sci & Technol, Chengdu 611130, Sichuan, Peoples R China
基金
中国国家自然科学基金;
关键词
Rhizobium anhuiense; symbiotic nodulation; seed exudates; lipopolysaccharide; transport genes; TRANSENVELOPE PROTEIN COMPLEX; ELECTROPHORETIC PROFILES; SP NGR234; STRAINS; LEGUMES;
D O I
10.1139/cjm-2019-0413
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipopolysaccharide (LPS) is essential for successful nodulation during the symbiosis of rhizobia and legumes. However, the detailed mechanism of the LPS in this process has not yet been clearly elucidated. In this study, the effects of common bean seed exudates on the growth, lipopolysaccharide production, and lipopolysaccharide transport genes expression (lpt) of Rhizobium anhuiense were investigated. Rhizobium anhuiense exposed to exudates showed changes in LPS electrophoretic profiles and content, whereby the LPS band was wider and the LPS content was higher in R. anhuiense treated with seed exudates. Exudates enhanced cell growth of R. anhuiense in a concentration-dependent manner; R. anhuiense exposed to higher doses of the exudate showed faster growth. Seven lpt genes of R. anhuiense were amplified and sequenced. Sequences of six lpt genes, except for lptE, were the same as those found in previously analyzed R. anhuiense strains, while lptE shared low sequence similarity with other strains. Exposure to the exudates strongly stimulated the expression of all lpt genes. Approximately 6.7- (lptG) to 301-fold (lptE) increases in the transcriptional levels were observed after only 15 min of exposure to exudates. These results indicate that seed exudates affect the LPS by making the cell wall structure more conducive to symbiotic nodulation.
引用
收藏
页码:186 / 193
页数:8
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