Force-Clamp Spectroscopy of Single Proteins

被引：5

作者：

Fernandez, Julio M. ^{[1
]}

Garcia-Manyes, Sergi ^{[1
]}

Dougan, Lorna ^{[1
]}

机构：

[1] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

来源：

SINGLE MOLECULE SPECTROSCOPY IN CHEMISTRY, PHYSICS AND BIOLOGY | 2010年 / 96卷

关键词：

TITIN IMMUNOGLOBULIN DOMAINS; MECHANICAL STABILITY; ENERGY LANDSCAPE; DYNAMICS; REVEALS; FIBRONECTIN; SIGNATURES; MOLECULES; UBIQUITIN; KINETICS;

D O I：

10.1007/978-3-642-02597-6_17

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Force-clamp AFM, with its remarkable ability to manipulate short recombinant proteins, has become a useful probe of protein dynamics, allowing us to sense conformational changes down to the sub-Angstrom scale. The single protein data is providing a new view that will help guide the development of theories on enzyme catalysis, the statistical dynamics of folding, and ab initio studies of a chemical reaction while placed under a stretching force; of common occurrence in nature.

引用

页码：317 / 335

页数：19

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