Kinetic Study of Candida antarctica Lipase B Immobilization Using Poly(Methyl Methacrylate) Nanoparticles Obtained by Miniemulsion Polymerization as Support

被引：23
|
作者：
Valerio, Alexsandra [1 ]
Nicoletti, Gabrieli [1 ]
Cipolatti, Eliane P. [1 ]
Ninow, Jorge L. [1 ]
Araujo, Pedro H. H. [1 ]
Sayer, Claudia [1 ]
de Oliveira, Debora [1 ]
机构：
[1] Univ Fed Santa Catarina, Chem Engn & Food Engn Dept, BR-88040900 Florianopolis, SC, Brazil
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2015年 / 175卷 / 06期
关键词：
Poly(methylmethacrylate); Miniemulsion; Nanoparticles; CalB enzyme; Lipase; ENCAPSULATION; HYDROPHOBE; STYRENE;
D O I：
10.1007/s12010-015-1478-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
With the objective to obtain immobilized Candida antarctica lipase B (CalB) with good activity and improved utilization rate, this study evaluated the influence of enzyme and crodamol concentrations and initiator type on the CalB enzyme immobilization in nanoparticles consisting of poly(methyl methacrylate) (PMMA) obtained by miniemulsion polymerization. The kinetic study of immobilized CalB enzyme in PMMA nanoparticles was evaluated in terms of monomer conversion, particle size, zeta potential, and relative activity. The optimum immobilization condition for CalB was compared with free enzyme in the p-NPL hydrolysis activity measurement. Results showed a higher CalB enzyme stability after 20 hydrolysis cycles compared with free CalB enzyme; in particular, the relative immobilized enzyme activity was maintained up to 40 %. In conclusion, PMMA nanoparticles proved to be a good support for the CalB enzyme immobilization and may be used as a feasible alternative catalyst in industrial processes.
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页码：2961 / 2971
页数：11
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