An improved and low-cost protocol for high-quality DNA isolation for the Chagas disease vectors

被引：1

作者：

David Cruz, Daryl ^{[1
]}

Nava-Garcia, Elizabeth ^{[2
]}

Arellano, Elizabeth ^{[1
]}

机构：

[1] Univ Autonoma Estado Morelos, Ctr Invest Biodiversidad & Conservac, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico

[2] Univ Autonoma Estado Morelos, Fac Biol, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico

来源：

INFECTION GENETICS AND EVOLUTION | 2020年 / 80卷

关键词：

DNA isolation; Genomic DNA; True bugs; Chagas disease; GENETIC-STRUCTURE; SPECIES LIMITS; MITOCHONDRIAL; REDUVIIDAE; SEQUENCE; COMPLEX;

D O I：

10.1016/j.meegid.2020.104201

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

An improved protocol for DNA extraction for the Chagas disease vectors is proposed based on modification to a low cost method described twenty years ago. Quality DNA and high molecular weight were obtained from all samples. NADH-4 gene was successfully amplified by PCR using the isolated DNA. The extraction protocol presented in this technical note is a fast, low-cost, and non-aggressive method to human health for obtaining genetic data from this group of epidemiological importance and potentially other insects.

引用

页数：4

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