An improved and low-cost protocol for high-quality DNA isolation for the Chagas disease vectors

被引：1
|
作者：
David Cruz, Daryl [1 ]
Nava-Garcia, Elizabeth [2 ]
Arellano, Elizabeth [1 ]
机构：
[1] Univ Autonoma Estado Morelos, Ctr Invest Biodiversidad & Conservac, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico
[2] Univ Autonoma Estado Morelos, Fac Biol, Av Univ 1001, Cuernavaca 62209, Morelos, Mexico
来源：
INFECTION GENETICS AND EVOLUTION | 2020年 / 80卷
关键词：
DNA isolation; Genomic DNA; True bugs; Chagas disease; GENETIC-STRUCTURE; SPECIES LIMITS; MITOCHONDRIAL; REDUVIIDAE; SEQUENCE; COMPLEX;
D O I：
10.1016/j.meegid.2020.104201
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
An improved protocol for DNA extraction for the Chagas disease vectors is proposed based on modification to a low cost method described twenty years ago. Quality DNA and high molecular weight were obtained from all samples. NADH-4 gene was successfully amplified by PCR using the isolated DNA. The extraction protocol presented in this technical note is a fast, low-cost, and non-aggressive method to human health for obtaining genetic data from this group of epidemiological importance and potentially other insects.
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页数：4
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