A micro system using dielectrophoresis and electrical impedance spectroscopy for cell manipulation and analysis

被引：0

作者：

Mohanty, SK ^{[1
]}

Ravula, SK ^{[1
]}

Engisch, KL ^{[1
]}

Frazier, AB ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Elect & Comp Engn, Atlanta, GA 30332 USA

来源：

BOSTON TRANSDUCERS'03: DIGEST OF TECHNICAL PAPERS, VOLS 1 AND 2 | 2003年

关键词：

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Single cell manipulation and analysis using dielectrophoresis (DEP) and micro electrical impedance spectroscopy (mu-EIS) has been demonstrated on bovine chromaffin cells and red blood cells. Cells were manipulated and analyzed on a micro scale electrophysiological analysis system fabricated using micromachining (e.g. MEMS) technologies. Cells were injected into a microreservoir and collected for mu-EIS analysis using vacuum and DEP. Impedance measurements were taken over a frequency range of 40 Hz to 3.0 MHz. The data obtained for the cells was compared to data obtained for air, and phosphate buffered solution. Data for chromaffin cells was analyzed further, assessing cell size and calcium ion channel activity in relation to impedance spectroscopy.

引用

页码：1055 / 1058

页数：4

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