Low frequency of endospore-specific genes in subseafloor sedimentary metagenomes

被引:8
|
作者
Kawai, Mikihiko [1 ]
Uchiyama, Ikuo [3 ,4 ]
Takami, Hideto [5 ]
Inagaki, Fumio [1 ,2 ]
机构
[1] Japan Agcy Marine Earth Sci & Technol JAMSTEC, Geomicrobiol Grp, Kochi Inst Core Sample Res, Nankoku, Kochi 7838502, Japan
[2] Japan Agcy Marine Earth Sci & Technol JAMSTEC, Geobiotechnol Grp, Res & Dev Ctr Submarine Resources, Nankoku, Kochi 7838502, Japan
[3] Natl Inst Nat Sci, Natl Inst Basic Biol, Okazaki, Aichi 4448585, Japan
[4] Grad Univ Adv Studies, Sch Life Sci, Dept Basic Biol, Okazaki, Aichi 4448585, Japan
[5] JAMSTEC, Inst Biogeosci, Microbial Genome Res Grp, Yokosuka, Kanagawa 2370061, Japan
来源
ENVIRONMENTAL MICROBIOLOGY REPORTS | 2015年 / 7卷 / 02期
基金
日本学术振兴会;
关键词
MARINE-SEDIMENTS; BACILLUS-MEGATERIUM; STATIONARY-PHASE; MICROBIAL LIFE; SEA; SPORULATION; DIVERSITY; ARCHAEA; RESISTANCE; BIOSPHERE;
D O I
10.1111/1758-2229.12254
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Spore formation is considered to be one of the microbial strategies for long-term survival in subseafloor sedimentary habitats. However, our knowledge of the genetic and physiological characteristics of subseafloor microbes is limited. Here, we studied the distribution and frequency of genes that are related to endospore formation in 10 subseafloor sedimentary metagenomes from Site C9001 off Japan and Site 1229 off Peru. None or very low frequencies of endospore-specific genes (e.g. dpaA, dpaB, sspA, spo0A, spoIIGA, spoIIM, spoIIIAB, spoIVA, spoIVB, yabP, yunB, spoVM) were observed in the subseafloor metagenomes. Based on the number of universally conserved single copy genes, the frequency ratio of putative endospore-formers was estimated to be <10%, which is consistent with the frequency of Clostridia-derived genomes (2-4%) but is lower than previous estimates based on the concentration of dipicolinic acid. Conceivable explanations for this discrepancy are as follows: the efficiency of lysis and DNA extraction of subseafloor endospore cells may have been lower than those of vegetative cells, conversion factor of dipicolinic acid content per cell may differ, and/or sporulation-related genes and other functional strategies for long-term survival in the deep subseafloor biosphere are evolutionarily distinct from known spore-forming gene repertoires.
引用
收藏
页码:341 / 350
页数:10
相关论文
共 50 条
  • [31] SPECIFIC FEATURES OF THE LOW-FREQUENCY VIBRATIONAL-SPECTRA OF CYLINDRICAL BODIES
    KUZNETSOV, ND
    FRIDMAN, LI
    SHAPOSHNIKOV, IN
    DOKLADY AKADEMII NAUK SSSR, 1990, 312 (01): : 55 - 58
  • [32] LOW POWER OF FAST FREQUENCY EEG IN AUTISM IS NOT SPECIFIC TO REM SLEEP IN AUTISM
    Duplan, S. M.
    Rochette, A.
    Limoges, E.
    Chevrier, E.
    Mottron, L.
    Godbout, R.
    SLEEP, 2010, 33 : A239 - A239
  • [33] Specific features in low-frequency vibrations of nanocrystals in fluorophosphate glassy matrices
    Denisov, EP
    Karpov, SV
    Kolobkova, EV
    Novikov, BV
    Suslikov, AI
    Fedorov, DL
    Yastrebova, MA
    PHYSICS OF THE SOLID STATE, 1999, 41 (07) : 1194 - 1197
  • [34] Specific features in low-frequency vibrations of nanocrystals in fluorophosphate glassy matrices
    E. P. Denisov
    S. V. Karpov
    E. V. Kolobkova
    B. V. Novikov
    A. I. Suslikov
    D. L. Fedorov
    M. A. Yastrebova
    Physics of the Solid State, 1999, 41 : 1194 - 1197
  • [35] Optogenetic Low-Frequency Stimulation of Specific Neuronal Populations Abates Ictogenesis
    Shiri, Zahra
    Levesque, XMaxime
    Etter, Guillaume
    Manseau, Frederic
    Williams, Sylvain
    Avoli, Massimo
    JOURNAL OF NEUROSCIENCE, 2017, 37 (11): : 2999 - 3008
  • [36] Low rates of expression profile divergence in highly expressed genes and tissue-specific genes during mammalian evolution
    Liao, Ben-Yang
    Zhang, Jianzhi
    MOLECULAR BIOLOGY AND EVOLUTION, 2006, 23 (06) : 1119 - 1128
  • [37] Profile of development to genes under extremely low frequency magnetic fields with caenorhabditis elegans
    Miyakawa, Tomokazu
    Harada, Shin-Ichi
    Ishimori, Takayuki
    Yamamoto, Hiroshi
    Hosono, Ryuji
    Yamada, Sotoshi
    International Journal of Applied Electromagnetics and Mechanics, 2001, 14 (1-4 SPEC.) : 323 - 326
  • [38] Profile of development to genes under extremely low frequency magnetic fields with caenorhabditis elegans
    Miyakawa, T
    Harada, S
    Ishimori, T
    Yamamoto, H
    Hosono, R
    Yamada, S
    INTERNATIONAL JOURNAL OF APPLIED ELECTROMAGNETICS AND MECHANICS, 2001, 14 (1-4) : 323 - 326
  • [39] Low-Frequency, Damaging Mutations in Hundreds of Genes Are Risk Factors for Endometriosis.
    Ward, Kenneth
    Ward, Kenneth
    Ward, Kenneth
    REPRODUCTIVE SCIENCES, 2017, 24 : 133A - 133A
  • [40] Frequency-Specific Alterations of Fractional Amplitude of Low-Frequency Fluctuations in Adult-Onset Hypothyroidism
    Shi, Qian
    Han, Yalan
    Tian, Jing
    Huang, Gang
    Tian, Limin
    Qin, Rui
    Cao, Jiancang
    Zhao, Lianping
    JOURNAL OF INTEGRATIVE NEUROSCIENCE, 2024, 23 (06)