Ubc9 fusion-directed SUMOylation (UFDS)

被引：9
|
作者：
Niedenthal, R. [1 ]
机构：
[1] Hannover Med Sch, Inst Physiol Chem, D-30625 Hannover, Germany
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 2007年 / 35卷
关键词：
E2 conjugating enzyme; protein modification; signal transducer and activator of transcription 1 (STAT1); small ubiquitin-related modifier (SUMO); SUMOylation-regulated phosphorylation; Ubc9 fusion-directed SUMOylation (UFDS); ubiquitin-conjugating enzyme 9 (Ubc9);
D O I：
10.1042/BST0351430
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
SUMOylation is a post-translational modification that is involved in the regulation of proteins of different cellular processes. Dependent on the transient, small SUMOylated portion of most target proteins, it is difficult to identify and characterize this modification and its functions, and it is even more difficult to study the interplay between SUMOylation and other modifications on a specific protein. To facilitate the analysis of protein SUMOylation and its interplay with other protein modifications, the UFDS (ubc9 fusion-directed SUMOylation) system has been developed. The identification of new SUMOylation substrates and the elucidation of the interplay between STAT1 (signal transducer and activator of transcription 1) phosphorylation and SUMOylation demonstrate UFDS as a useful tool for analysing protein SUMOylation.
引用
收藏
页码：1430 / 1432
页数：3
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