Pib2 and the EGO complex are both required for activation of TORC1

被引：30
|
作者：
Varlakhanova, Natalia V. [1 ]
Mihalevic, Michael J. [2 ]
Bernstein, Kara A. [2 ]
Ford, Marijn G. J. [1 ]
机构：
[1] Univ Pittsburgh, Sch Med, Dept Cell Biol & Physiol, 3500 Terrace St, Pittsburgh, PA 15261 USA
[2] Univ Pittsburgh, Sch Med, Dept Microbiol & Mol Genet, 5117 Ctr Ave, Pittsburgh, PA 15213 USA
来源：
JOURNAL OF CELL SCIENCE | 2017年 / 130卷 / 22期
基金：
美国国家卫生研究院;
关键词：
EGO complex; Gtr1; Gtr2; Pib2; TORC1; TRANSFER-RNA SYNTHETASE; SACCHAROMYCES-CEREVISIAE; PROTEIN COMPLEXES; CRYSTAL-STRUCTURE; GROWTH-CONTROL; CELL-GROWTH; RAG GTPASES; YEAST; PATHWAY; KINASE;
D O I：
10.1242/jcs.207910
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The TORC1 complex is a key regulator of cell growth and metabolism in Saccharomyces cerevisiae. The vacuole-associatedEGO complex couples activation of TORC1 to the availability of amino acids, specifically glutamine and leucine. The EGO complex is also essential for reactivation of TORC1 following rapamycin-induced growth arrest and for its distribution on the vacuolar membrane. Pib2, a FYVE-containing phosphatidylinositol 3-phosphate (PI3P)-binding protein, is a newly discovered and poorly characterized activator of TORC1. Here, we show that Pib2 is required for reactivation of TORC1 following rapamycin-induced growth arrest. Pib2 is required for EGO complex-mediated activation of TORC1 by glutamine and leucine as well as for redistribution of Tor1 on the vacuolar membrane. Therefore, Pib2 and the EGO complex cooperate to activate TORC1 and connect phosphoinositide 3-kinase (PI3K) signaling and TORC1 activity.
引用
收藏
页码：3878 / 3890
页数：13
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