Integrated Phenotypic-Genotypic Analysis of Latilactobacillus sakei from Different Niches

被引：12
|
作者：
Chen, Ying [1 ]
Li, Nan [2 ]
Zhao, Shenxi [1 ]
Zhang, Chuan [1 ]
Qiao, Nanzhen [3 ]
Duan, Hui [1 ]
Xiao, Yue [1 ]
Yan, Bowen [1 ,4 ]
Zhao, Jianxin [1 ,4 ]
Tian, Fengwei [1 ,4 ]
Zhai, Qixiao [1 ,4 ]
Yu, Leilei [1 ,4 ]
Chen, Wei [1 ,4 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Bright Dairy & Food Co Ltd, State Key Lab Dairy Biotechnol, Shanghai Engn Res Ctr Dairy Biotechnol, Dairy Res Inst, Shanghai 200436, Peoples R China
[3] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2R3, Canada
[4] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Jiangsu, Peoples R China
来源：
FOODS | 2021年 / 10卷 / 08期
基金：
中国国家自然科学基金;
关键词：
Latilactobacillus sakei; comparative genomics; carbohydrate utilization; antibiotic tolerance; CRISPR-Cas; LACTOBACILLUS-SAKEI; LISTERIA-MONOCYTOGENES; ACQUIRED-RESISTANCE; SEQUENCE; GENES; TETRACYCLINE; BACTERIOCIN; DATABASE; GENOMES; K040706;
D O I：
10.3390/foods10081717
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Increasing attention has been paid to the potential probiotic effects of Latilactobacillus sakei. To explore the genetic diversity of L. sakei, 14 strains isolated from different niches (feces, fermented kimchi, and meat products) and 54 published strains were compared and analyzed. The results showed that the average genome size and GC content of L. sakei were 1.98 Mb and 41.22%, respectively. Its core genome mainly encodes translation and transcription, amino acid synthesis, glucose metabolism, and defense functions. L. sakei has open pan-genomic characteristics, and its pan-gene curve shows an upward trend. The genetic diversity of L. sakei is mainly reflected in carbohydrate utilization, antibiotic tolerance, and immune/competition-related factors, such as clustering regular interval short palindromic repeat sequence (CRISPR)-Cas. The CRISPR system is mainly IIA type, and a few are IIC types. This work provides a basis for the study of this species.
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页数：18
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