Electrochemical Determination of Baicalein, Baicalin and Quercetin in Scutellaria barbata

Scutellariae barbata, a Chinese traditional medicine plant, are composed of three important components ( Baicalein, baicalin and quercetin). Nowadays, to develop a simple technique for determining the containing Baicalein, baicalin and quercetin accurately and economically is highly demanded. Herein, capillary electrophoresis ( CE) was incorporated with electrochemical detection ( ED) to provide more sensitive and selective determination method. Baicalein, baicalin and quercetin could be well separated within 12 min using the proposed CE-ED method with the optimum conditions. The detection limits ( S/N=3) was in the concentration range of 0.214 to 0.495 mu M for all three analysts. Owing to the high resolution, good reproducibility and excellent stability, the CE-ED method can be successfully employed for the determination of actual samples.