Orthogonal potency analysis of mesenchymal stromal cell function during ex vivo expansion

被引:9
|
作者
Khong, Danika [1 ,2 ]
Li, Matthew [1 ,2 ]
Singleton, Amy [1 ,2 ]
Chin, Ling-Yee [1 ,2 ]
Mukundan, Shilpaa [1 ,2 ]
Parekkadan, Biju [1 ,2 ,3 ,4 ,5 ]
机构
[1] Harvard Med Sch, Massachusetts Gen Hosp, Ctr Surg Innovat & Bioengn, Dept Surg, Boston, MA 02114 USA
[2] Shriners Hosp Children, Boston, MA 02114 USA
[3] Rutgers State Univ, Dept Biomed Engn, Piscataway, NJ 08854 USA
[4] Rutgers Biomed & Hlth Sci, Dept Med, Piscataway, NJ 08854 USA
[5] Harvard Stem Cell Inst, Cambridge, MA 02138 USA
基金
美国国家卫生研究院;
关键词
Mesenchymal stromal cell; Aging; Cell morphology; Adipogenesis; Hematopoiesis: Immuno-suppression; HEMATOPOIETIC STEM-CELLS; NUCLEAR-PROTEIN NUMA; VERSUS-HOST-DISEASE; BONE-MARROW; IN-VITRO; PRECURSOR CELLS; PROLIFERATION; HETEROGENEITY; INFLAMMATION; TRANSPLANTS;
D O I
10.1016/j.yexcr.2017.11.007
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Adult bone marrow mesenchymal stromal cells (MSCs) have cross-functional, intrinsic potency that is of therapeutic interest. Their ability to regenerate bone, fat, and cartilage, modulate the immune system, and nurture the growth and function of other bone marrow hematopoietic stem/progenitor cells have all been evaluated by transplant applications of MSCs. These applications require the isolation and expansion scaled cell production. To investigate biophysical properties of MSCs that can be feasibly utilized as predictors of bioactivity during biomanufacturing, we used a low-density seeding model to drive MSCs into proliferative stress and exhibit the hallmark characteristics of in vitro aging. A low-density seeding method was used to generate MSCs from passages 1-7 to simulate serial expansion of these cells to maximize yield from a single donor. MSCs were subjected to three bioactivity assays in parallel to ascertain whether patterns in MSC age, size, and shape were associated with the outcomes of the potency assays. MSC age was found to be a predictor of adipogenesis, while cell and nuclear shape was strongly associated to hematopoietic-supportive potency. Together, these data evaluate morphological changes associated with cell potency and highlight new strategies for purification or alternatives to assessing MSC quality.
引用
收藏
页码:102 / 110
页数:9
相关论文
共 50 条
  • [31] Ex vivo purging and cell expansion
    Ball, ED
    JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH, 1999, 8 (05): : 457 - 458
  • [32] Human amniotic mesenchymal stromal cells support the ex vivo expansion of cord blood hematopoietic stem cells
    Orticelli, Valentina
    Papait, Andrea
    Vertua, Elsa
    Bonassi Signoroni, Patrizia
    Romele, Pietro
    Di Pietro, Lorena
    Magatti, Marta
    Teofili, Luciana
    Silini, Antonietta Rosa
    Parolini, Ornella
    STEM CELLS TRANSLATIONAL MEDICINE, 2021, 10 (11) : 1516 - 1529
  • [33] Adult ovine connective tissue cells resemble mesenchymal stromal cells in their propensity for extensive ex vivo expansion
    Linde, Peter E.
    Puttlitz, Christian M.
    Kisiday, John D.
    CONNECTIVE TISSUE RESEARCH, 2021, 62 (06) : 671 - 680
  • [34] Bone marrow derived mesenchymal stromal cells promote treg cell development ex vivo
    Yao, Qingping
    Wang, James
    Singh, Ram Raj
    CLINICAL IMMUNOLOGY, 2007, 123 : S106 - S106
  • [35] Encapsulated Mesenchymal Stromal Cell Microbeads Promote Endogenous Regeneration of Osteoarthritic Cartilage Ex Vivo
    Sahu, Neety
    Agarwal, Pranay
    Grandi, Fiorella
    Bruschi, Michela
    Goodman, Stuart
    Amanatullah, Derek
    Bhutani, Nidhi
    ADVANCED HEALTHCARE MATERIALS, 2021, 10 (08)
  • [36] Human Mesenchymal Stromal Cells Enhance the Stability of Umbilical Cord Blood Inducible Tregs during Ex Vivo Expansion Via Mitochondria Transfer
    Do, Jeong Su
    Zwick, Daniel
    Huang, Alex Yee-Chen
    Zhong, Fei
    Askew, David
    Finney, Marcie
    Van't Hof, Wouter
    Laughlin, Mary J.
    BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, 2019, 25 (03)
  • [37] Cytogenetic evaluation of mesenchymal stem/stromal cells from patients with myelodysplastic syndromes at different time-points during ex vivo expansion
    Kouvidi, Elisavet
    Stratigi, Aikaterini
    Batsali, Aristea
    Mavroudi, Irene
    Mastrodemou, Semeli
    Ximeri, Maria
    Papadaki, Helen A.
    Pontikoglou, Charalampos G.
    LEUKEMIA RESEARCH, 2016, 43 : 24 - 32
  • [38] QUALITY DEVELOPMENT OF MESENCHYMAL STROMAL CELL MANUFACTURING BASED ON POTENCY ANALYSIS OF CELLULAR SECRETOMES
    Koaykul, C.
    CYTOTHERAPY, 2024, 26 (06) : S51 - S51
  • [39] Ex vivo analysis of T-cell function
    Suni, MA
    Maino, VC
    Maecker, HT
    CURRENT OPINION IN IMMUNOLOGY, 2005, 17 (04) : 434 - 440
  • [40] Immunophenotypical Characterization of Limbal Mesenchymal Stromal Cell Subsets during In Vitro Expansion
    Aghazadeh, Sara
    Peng, Qiuyue
    Dardmeh, Fereshteh
    Hjortdal, Jesper Ostergaard
    Zachar, Vladimir
    Alipour, Hiva
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (16)