Two-photon excitation of fluorescence from chicken tissue

被引:22
|
作者
Guo, YC
Wang, QZ
Zhadin, N
Liu, F
Demos, S
Calistru, D
Tirksliunas, A
Katz, A
Budansky, Y
Ho, PP
Alfano, RR
机构
[1] CUNY CITY COLL, DEPT PHYS, INST ULTRAFAST SPECT & LASERS, NEW YORK, NY 10031 USA
[2] CUNY CITY COLL, NEW YORK STATE CTR ADV TECHNOL ULTRAFAST PHOTON M, NEW YORK, NY 10031 USA
[3] CUNY GRAD SCH & UNIV CTR, NEW YORK, NY 10031 USA
关键词
two-photon excitation-induced fluorescence; tissue; spectroscopy;
D O I
10.1364/AO.36.000968
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We have measured UV fluorescence excited through two-photon absorption from native chicken tissue, using 600-nm, 500-fs pulses from a R6G dye laser. The observed emission signal was found to depend quadratically on the excitation intensity. The two-photon excitation-induced fluorescence spectrum is attributed to tryptophan residues in proteins. (C) 1997 Optical Society of America
引用
收藏
页码:968 / 970
页数:3
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