Two-photon excitation of fluorescence from chicken tissue

被引:22
|
作者
Guo, YC
Wang, QZ
Zhadin, N
Liu, F
Demos, S
Calistru, D
Tirksliunas, A
Katz, A
Budansky, Y
Ho, PP
Alfano, RR
机构
[1] CUNY CITY COLL, DEPT PHYS, INST ULTRAFAST SPECT & LASERS, NEW YORK, NY 10031 USA
[2] CUNY CITY COLL, NEW YORK STATE CTR ADV TECHNOL ULTRAFAST PHOTON M, NEW YORK, NY 10031 USA
[3] CUNY GRAD SCH & UNIV CTR, NEW YORK, NY 10031 USA
关键词
two-photon excitation-induced fluorescence; tissue; spectroscopy;
D O I
10.1364/AO.36.000968
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We have measured UV fluorescence excited through two-photon absorption from native chicken tissue, using 600-nm, 500-fs pulses from a R6G dye laser. The observed emission signal was found to depend quadratically on the excitation intensity. The two-photon excitation-induced fluorescence spectrum is attributed to tryptophan residues in proteins. (C) 1997 Optical Society of America
引用
收藏
页码:968 / 970
页数:3
相关论文
共 50 条
  • [1] Two-photon excitation fluorescence bioassays
    Hanninen, Pekka
    Soukka, Jori
    Soini, Juhani T.
    [J]. FLUORESCENCE METHODS AND APPLICATIONS: SPECTROSCOPY, IMAGING, AND PROBES, 2008, 1130 : 320 - 326
  • [2] Two-photon excitation fluorescence microscopy
    So, PTC
    Dong, CY
    Masters, BR
    Berland, KM
    [J]. ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2000, 2 : 399 - 429
  • [3] Fluorescence spectroscopy of biological tissue: single- and two-photon excitation
    Wu, YC
    Xi, P
    Ge, WK
    Yuen, PW
    Qu, JY
    [J]. MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES IV, 2004, 5323 : 377 - 381
  • [4] Two-color two-photon excitation of fluorescence
    Lakowicz, JR
    Gryczynski, I
    Malak, H
    Gryczynski, Z
    [J]. ADVANCES IN FLUORESCENCE SENSING TECHNOLOGY III, 1997, 2980 : 368 - 380
  • [5] Two-color two-photon excitation of fluorescence
    Lakowicz, JR
    Gryczynski, I
    Malak, H
    Gryczynski, Z
    [J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1996, 64 (04) : 632 - 635
  • [6] Chromatic two-photon excitation fluorescence imaging
    Xu, Q.
    Shi, K.
    Yin, S.
    Liu, Z.
    [J]. JOURNAL OF MICROSCOPY, 2009, 235 (01) : 79 - 83
  • [7] Two-photon excitation in fluorescence lifetime imaging
    Gerritsen, HC
    Vroom, J
    Sytsma, J
    [J]. FLUORESCENCE MICROSCOPY AND FLUORESCENT PROBES, VOL 2, 1998, : 55 - 62
  • [8] Two-photon fluorescence excitation in detection of biomolecules
    Soini, E
    Meltola, NJ
    Soini, AE
    Soukka, J
    Soini, JT
    Hänninen, PE
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 2000, 28 : 70 - 74
  • [9] THE TWO-PHOTON FLUORESCENCE EXCITATION SPECTRUM OF DIBENZOTHIOPHENE
    CASTELLUCCI, E
    FOGGI, P
    SALVI, PR
    [J]. CHEMICAL PHYSICS, 1981, 63 (03) : 437 - 443
  • [10] Two-color two-photon excitation of fluorescence.
    Lakowicz, JR
    Gryczynski, I
    Gryczynski, Z
    Malak, H
    [J]. BIOPHYSICAL JOURNAL, 1997, 72 (02) : MP377 - MP377