Staphylococcal enterotoxin a detection with phage displayed antibodies

被引：3
|
作者：
Fursova, K. K. [1 ,2 ]
Shchannikova, M. P. [1 ,2 ]
Shepelyakovskaya, A. O. [1 ,2 ]
Pavlik, L. L. [3 ]
Brovko, F. A. [1 ,2 ]
机构：
[1] Ernst Inst Anim Husb, Podolsk Dist 142132, Moscow Oblast, Russia
[2] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Pushchino Branch, Pushchino 142290, Moscow Oblast, Russia
[3] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142290, Moscow Oblast, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2016年 / 42卷 / 03期
基金：
俄罗斯科学基金会;
关键词：
Staphylococcal enterotoxin A; phage displayed antibodies; sandwich enzyme-linked immunosorbent assay; AGGREGATION; ARGININE; AUREUS; TOXINS; SUPPRESSION; OUTBREAK; DOMAINS; BINDING;
D O I：
10.1134/S1068162016020035
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A technique for detection of staphylococcal enterotoxin A with sandwich enzyme-linked immunoassay (ELISA) was developed. Mouse monoclonal anti-SEA antibodies were used as capture antibodies and phage displayed anti-SEA scFv were used as detection antibodies. The limit of detection was 6-12.5 ng/mL for different pairs of antibodies. Some conditions of phage-displayed antibodies for storage in dissolved and lyophilized state were examined. It was shown the use of trehalose and arginine preserved the functional activity of phage-displayed antibodies.
引用
收藏
页码：294 / 299
页数：6
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