Staphylococcal enterotoxin a detection with phage displayed antibodies

被引：3

作者：

Fursova, K. K. ^{[1
,2
]}

Shchannikova, M. P. ^{[1
,2
]}

Shepelyakovskaya, A. O. ^{[1
,2
]}

Pavlik, L. L. ^{[3
]}

Brovko, F. A. ^{[1
,2
]}

机构：

[1] Ernst Inst Anim Husb, Podolsk Dist 142132, Moscow Oblast, Russia

[2] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Pushchino Branch, Pushchino 142290, Moscow Oblast, Russia

[3] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142290, Moscow Oblast, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2016年 / 42卷 / 03期

基金：

俄罗斯科学基金会;

关键词：

Staphylococcal enterotoxin A; phage displayed antibodies; sandwich enzyme-linked immunosorbent assay; AGGREGATION; ARGININE; AUREUS; TOXINS; SUPPRESSION; OUTBREAK; DOMAINS; BINDING;

D O I：

10.1134/S1068162016020035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A technique for detection of staphylococcal enterotoxin A with sandwich enzyme-linked immunoassay (ELISA) was developed. Mouse monoclonal anti-SEA antibodies were used as capture antibodies and phage displayed anti-SEA scFv were used as detection antibodies. The limit of detection was 6-12.5 ng/mL for different pairs of antibodies. Some conditions of phage-displayed antibodies for storage in dissolved and lyophilized state were examined. It was shown the use of trehalose and arginine preserved the functional activity of phage-displayed antibodies.

引用

页码：294 / 299

页数：6

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