Radiation-induced p53 and p21WAF-1/CIP1 expression in the murine intestinal epithelium -: Apoptosis and cell cycle arrest

p53-dependent expression of p21(WAF-1/CIP1) has been studied in murine intestinal epithelium after exposure to ionizing radiation. In un-irradiated small intestine, neither p55 nor p21(WAF-1/CIP1) could be detected by immunohistochemistry. After irradiation (8 Gy), there was a time- and dose-dependent increase in the expression of both proteins. In the small bowel, the positional expression of p53 and p21(WAF-1/CIP1) was similar but not coincident. Both proteins could be observed throughout the crypts with greatest frequency of expression over the first 15 cell positions, which includes the stem cell population (approximately positions 3 to 5) and the proliferating, transit cell population (approximately positions 5 to 15), p53-positive cells were primarily distributed toward the base of the crypt relative to p21(WAF-1/CIP1). Subdivision of the p53-positive cell population revealed that the cells with strongest p53 immunoreactivity were positioned farther toward the base of the crypt, and their distribution was approximately coincident with the frequency distribution of apoptotic cells. Cells that were either weakly or moderately immunoreactive for p53 were located toward the middle of the crypt and were approximately coincident with the distribution of p21(WAF-1/CIP1). The numbers of both p53- and p21(WAF-1/CIP1)-positive cells declined steadily with time, and by 6 days after irradiation there were very few immunoreactive cells to observe. Radiation-induced increase in p53 and p21(WAF-1/CIP1) expression was not detected in mice homozygously null for p53. Expression of p21(WAF-1/CIP1) and incorporation of tritiated thymidine were found to be mutually exclusive. In the large bowel, p21(WAF-1/CIP1) and p53 expression were observed along the entire length of the colonic crypts after irradiation (8 Gy), and, unlike in the small intestine, this expression was not only maintained but increased over 72 hours. p21(WAF-1/CIP1) immunoreactivity was detected in large intestine epithelium up to 6 days after irradiation, The differential expression of p21(WAF-1/CIP1), observed between the large and small bowel and within the small intestinal crypts, is discussed.