Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen (PSA) Based on Graphene-Silver Nanocomposite

被引：4
|
作者：
Yu, Lei [1 ]
Fang, Zhen [2 ]
Li, Wenxian [3 ]
Li, Shenqian [1 ]
Wang, Peitao [1 ]
Wang, Hongqiang [1 ]
机构：
[1] Qingdao Univ, Dept Androl, Affiliated Hosp, 16 Jiangsu Rd, Qingdao, Peoples R China
[2] Qingdao Haici Med Grp, Dept Ophthalmol, Qingdao 266000, Shandong, Peoples R China
[3] Qingdao Univ, Dept Urol, Affiliated Hosp, 16 Jiangsu Rd, Qingdao, Shandong, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2017年 / 12卷 / 09期
关键词：
Prostate-specific antigen; Electrochemical sensor; Graphene; Silver; Children asthmatic Layer-by-layer; IMMUNOSENSOR; IMMUNOASSAY; BIOSENSOR; SENSOR; STRATEGY; ASSAY;
D O I：
10.20964/2017.09.62
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
In this work, the electrostatic layer-by-layer (LbL) assembly of bovine serum albumin (BSA) functionalized graphene nanosheets and silver nanoparticle (AgNP), along with the thermal annealing at 340 degrees C in air were performed to prepare a thin and uniform film of AgNP embedded porous graphene (AgEPG). To determine prostate specific antigen (PSA) in human serum, we proposed a new electrochemical immunosensor based on a protein tracer, namely the functionalized AgEPG. Our proposed immunosensor was feasible to detect PSA (1-35 ng/mL) under optimal electrochemical and immunoreactions determination conditions, and the limit of detection (LOD) was calculated as 0.44 ng/mL.
引用
收藏
页码：8188 / 8197
页数：10
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