Continuous wave two-photon scanning near-field optical microscopy

被引：20

作者：

Kirsch, AK ^{[1
]}

Subramaniam, V ^{[1
]}

Striker, G ^{[1
]}

Schnetter, C ^{[1
]}

Arndt-Jovin, DJ ^{[1
]}

Jovin, TM ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Mol Biol, D-37077 Gottingen, Germany

来源：

BIOPHYSICAL JOURNAL | 1998年 / 75卷 / 03期

关键词：

D O I：

10.1016/S0006-3495(98)74070-6

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have implemented continuous-wave two-photon excitation of near-UV absorbing fluorophores in a scanning near-field optical microscope (SNOM). The 647-nm emission of an Ar-Kr mixed gas laser was used to excite the UV-absorbing DNA dyes DAPI, the bisbenzimidazole Hoechst 33342, and ethidium bromide in a shared aperture SNOM with uncoated fiber tips. Polytene chromosomes of Drosophila melanogaster and the nuclei of 3T3 Balb/c cells labeled with these dyes were readily imaged. The fluorescence intensity showed the expected nonlinear (second order) dependence on the excitation power in the range of 8-180 mW. We measured the fluorescence intensity as a function of the tip-sample displacement in the direction normal to the sample surface in the single- and two-photon excitation modes (SPE, TPE). The fluorescence intensity decayed faster in TPE than in SPE.

引用

页码：1513 / 1521

页数：9

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