Development of fifty-one novel EST-SSR loci for use in rockfish (genus Sebastes)

被引：1
|
作者：
Martinez, Andres N. [1 ]
Aguilar, Andres [1 ]
机构：
[1] Univ Calif Mered, Sch Nat Sci, Merced, CA 95344 USA
来源：
CONSERVATION GENETICS RESOURCES | 2011年 / 3卷 / 02期
关键词：
Sebastes; EST-SSR; Genomics; Stock identification; PROGRAM;
D O I：
10.1007/s12686-010-9357-4
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
We mined expressed sequence tag libraries from three species of rockfish (Sebastes caurinus, S. goodei, and S. rastrelliger) for microsatellites. A total of 111 novel sequences with repetitive elements were isolated and we were able to successfully amplify 51 of these in five different rockfish species. Population level surveys for two species (S. flavidus and S. melanops) were conducted with these 51 loci. Twelve loci showed deviations from HardyWeinberg equilibrium, indicating the presence of null alleles or duplicated loci. Eleven of the 51 loci annotated to the SWISSPROT database. This suite of loci will prove useful in current and ongoing conservation and genomic studies of rockfish.
引用
下载
收藏
页码：335 / 340
页数：6
相关论文
共 50 条
  • [41] Transcriptome Analysis and Novel EST-SSR Marker Development for Pinus tabuliformis Seedlings from Four Provenances
    Wang, Ju
    Guo, Shuai
    Zhang, Yongxin
    Zhang, Feng
    Yun, Yufei
    Zhang, Guosheng
    FORESTS, 2023, 14 (09):
  • [42] Development and Characterization of Novel EST-SSR Markers in Masson Pine (Pinus massoniana) Based on Transcriptome Data
    Pan, Xiaocheng
    Hu, Haibo
    RUSSIAN JOURNAL OF GENETICS, 2021, 57 (05) : 561 - 567
  • [43] Development and use of EST-SSR markers for assessing genetic diversity in the brown planthopper (Nilaparvata lugens Stal)
    Jing, S.
    Liu, B.
    Peng, L.
    Peng, X.
    Zhu, L.
    Fu, Q.
    He, G.
    BULLETIN OF ENTOMOLOGICAL RESEARCH, 2012, 102 (01) : 113 - 122
  • [44] Characterization and development of transcriptome-derived novel EST-SSR markers to assess genetic diversity in Chaetomium globosum
    K. Darshan
    Rashmi Aggarwal
    Bishnu Maya Bashyal
    Jagmohan Singh
    M. S. Saharan
    M. S. Gurjar
    Amolkumar U. Solanke
    3 Biotech, 2023, 13
  • [45] Characterization and development of transcriptome-derived novel EST-SSR markers to assess genetic diversity in Chaetomium globosum
    Darshan, K.
    Aggarwal, Rashmi
    Bashyal, Bishnu Maya
    Singh, Jagmohan
    Saharan, M. S.
    Gurjar, M. S.
    Solanke, Amolkumar U.
    3 BIOTECH, 2023, 13 (11)
  • [46] Development of novel EST-SSR markers for ploidy identification based on de novo transcriptome assembly for Misgurnus anguillicaudatus
    Feng, Bing
    Yi, Soojin V.
    Zhang, Manman
    Zhou, Xiaoyun
    PLOS ONE, 2018, 13 (04):
  • [47] Development of novel EST-SSR markers for Phyllanthus emblica (Phyllanthaceae) and cross-amplification in two related species
    Liu, Xiongfang
    Ma, Hong
    Li, Taiqiang
    Li, Zhenghong
    Wan, Youming
    Liu, Xiuxian
    Fu, Liyong
    APPLICATIONS IN PLANT SCIENCES, 2018, 6 (07):
  • [48] Characterization and novel Est-SSR marker development of an important Chinese medicinal plant, Morinda officinalis How (Rubiaceae)
    Liao, Boyong
    Lee, Shiouyih
    Meng, Kaikai
    Yin, Qianyi
    Huang, Cuiying
    Fan, Qiang
    Liao, Wenbo
    Chen, Sufang
    BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2019, 33 (01) : 1311 - 1318
  • [49] Development and characterization of 60 novel EST-SSR markers in half-smooth tongue sole Cynoglossus semilaevis
    Sha, Z-X.
    Luo, X-H.
    Liao, X-L.
    Wang, S-L.
    Wang, Q-L.
    Chen, S-L.
    JOURNAL OF FISH BIOLOGY, 2011, 78 (01) : 322 - 331
  • [50] Development and characterization of cDNA library based novel EST-SSR marker in radish (Raphanus sativus L.)
    Jiang, Lina
    Wang, Liangju
    Liu, Liwang
    Zhu, Xianwen
    Zhai, Lulu
    Cong, Yiqin
    SCIENTIA HORTICULTURAE, 2012, 140 : 164 - 172
12345