A PCR-based assay to detect hAT-like transposon sequences in plants

被引：7
|
作者：
De Keukeleire, P
De Schepper, S
Gielis, J
Gerats, T
机构：
[1] State Univ Ghent, Fac Sci, Dept Plant Syst Biol, B-9000 Ghent, Belgium
[2] Geniaal Bvba, B-2018 Antwerp, Belgium
[3] State Univ Ghent, Fac Appl Biol & Agr Sci, Dept Plant Prod, B-9000 Ghent, Belgium
来源：
CHROMOSOME RESEARCH | 2004年 / 12卷 / 02期
关键词：
hAT-like transposon; phylogeny; plant genomics; polymerase chain reaction (PCR);
D O I：
10.1023/B:CHRO.0000013163.34505.96
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Despite their potential as endogenous tools for forward and reverse genetics, members of the hobo, Ac, Tam3 (or hAT) superfamily of transposable elements have been characterized in but a limited number of plant species. To expedite their isolation, we developed a PCR-based assay for the detection of hAT-like transposon sequences in plants which was applied to isolate and initially characterize such sequences from Petunia hybrida, Phaseolus vulgaris, Bambusa vulgaris, Brassica napus and Rhododendron simsii.
引用
收藏
页码：117 / 123
页数：7
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