LC-MS/MS method for the quantification of myo- and chiro-inositol as the urinary biomarkers of insulin resistance in human urine

被引：6

作者：

Kim, Byoung-Hyoun ^{[1
]}

Park, Jin-Young ^{[2
]}

Jang, Jae Bum ^{[2
]}

Moon, Dong Cheul ^{[3
]}

机构：

[1] LG Chem Ltd, Analyt Sci, Taejon 305380, South Korea

[2] Hoseo Univ, Reg Innovat Ctr, Baebang Eup 336795, Asan, South Korea

[3] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, South Korea

来源：

BIOMEDICAL CHROMATOGRAPHY | 2012年 / 26卷 / 04期

关键词：

myo-inositol; chiro-inositol; urinary biomarker; LC-MS; MS; method validation; LIQUID-CHROMATOGRAPHY; MYOINOSITOL;

D O I：

10.1002/bmc.1682

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple LC-MS/MS method has been developed and validated for the quantification of endogenous myo- and chiro-inositol in human urine. myo- and chiro-Inositol were completely resolved from other carbohydrates and there were no interference peaks in human urine. The correlation coefficient (n?=?3) was greater than 0.9991 over the range 0.0525.0?mu g/mL with the weighted (1/C2) least square method. Precision (%RSD) and accuracy (%RE) were 010.0% and 06.0% for the intra-day assay (n?=?5) and 014.3% and 010.0% for the inter-day assay (n?=?5). myo- and chiro-Inositol have been shown to be stable in human urine stored at room temperature and for three freezethaw cycles. Copyright (c) 2011 John Wiley & Sons, Ltd.

引用

页码：429 / 433

页数：5

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