Fabrication of submicron proteinaceous structures by direct laser writing

被引：20

作者：

Serien, Daniela ^{[1
]}

Takeuchi, Shoji ^{[1
,2
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Ctr Int Res Integrat Biomed Syst, Meguro Ku, Tokyo 1538505, Japan

[2] Japan Sci & Technol Agcy, ERATO Takeuchi Biohybrid Innovat Project, Meguro Ku, Tokyo 1538505, Japan

来源：

APPLIED PHYSICS LETTERS | 2015年 / 107卷 / 01期

关键词：

CROSS-LINKING; MULTIPHOTON LITHOGRAPHY; CELL-CULTURE; MICROSTRUCTURES; MICROFABRICATION; POLYMERIZATION; DNA;

D O I：

10.1063/1.4926659

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

In this paper, we provide a characterization of truly free-standing proteinaceous structures with submicron feature sizes depending on the fabrication conditions by model-based analysis. Protein cross-linking of bovine serum albumin is performed by direct laser writing and two-photon excitation of flavin adenine dinucleotide. We analyze the obtainable fabrication resolution and required threshold energy for polymerization. The applied polymerization model allows prediction of fabrication conditions and resulting fabrication size, alleviating the application of proteinaceous structure fabrication. (C) 2015 AIP Publishing LLC.

引用

页数：5

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