Human apolipoproteins A-I and A-II in cell cholesterol efflux - Studies with transgenic mice

被引:43
|
作者
Chiesa, G
Parolini, C
Canavesi, M
Colombo, N
Sirtori, CR
Fumagalli, R
Franceschini, G
Bernini, F
机构
[1] Univ Parma, Inst Pharmacol & Pharmacognosy, I-43100 Parma, Italy
[2] Univ Milan, Inst Pharmacol Sci, Milan, Italy
[3] Univ Milan, Ctr E Grossi Paoletti, Milan, Italy
关键词
reverse cholesterol transport; HDL; tissue culture cells; phospholipids; atherosclerosis;
D O I
10.1161/01.ATV.18.9.1417
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The first step in reverse cholesterol transport is the movement of cholesterol out of cells onto lipoprotein accepters in the interstitial fluid. The contribution of specific lipoprotein components to this process remains to be established. In this study, the role of human apolipoproteins (apo) A-I and A-II in the efflux of cellular cholesterol was investigated in transgenic mouse models in which the expression of murine apoA-I was abolished due to gene targeting (A-IKO). Serum from A-IKO mice and from mice expressing human apoA-I and/or human apoA-II was incubated with [H-3]cholesterol-labeled Fu5AH rat hepatoma cells for 4 hours at 37 degrees C. The cholesterol efflux to the serum of A-IKO mice was markedly lower than that to the serum of mice transgenic for human apoA-I (5.0+/-1.5% versus 25.0+/-4.0%). Expression of human apoA-II alone did not modify the cholesterol efflux capacity of A-IKO mouse serum. Cholesterol efflux to serum of mice expressing human apoA-II together with human apoA-I was significantly lower than that to human apoA-I mouse serum (20.0+/-12.3% versus 25.0+/-4.0%). Regression analysis of cholesterol efflux versus the lipid/apolipoprotein concentrations of mouse serum suggested that 3 independent factors contribute to determine the cholesterol efflux potential of serum: the apolipoprotein composition of HDL, the serum concentration of HDL phospholipids, and the presence of a small fraction of particles containing apoA-I.
引用
收藏
页码:1417 / 1423
页数:7
相关论文
共 50 条
  • [31] Cholesterol homeostatic mechanisms in apo A-II expressing transgenic mice
    Kalopissis, AD
    Pastier, D
    Boisfer, E
    Atger, V
    Laplaud, M
    Quang, NT
    Griglio, S
    Chambaz, J
    ATHEROSCLEROSIS, 1999, 144 : 164 - 164
  • [32] EVIDENCE THAT OVEREXPRESSION OF HUMAN APOLIPOPROTEIN A-II IN TRANSGENIC MICE MODULATES REVERSE CHOLESTEROL TRANSPORT
    TAILLEUX, A
    CASTRO, G
    FIEVET, C
    TREMP, G
    TOUCHET, N
    DEGEITERE, C
    DELFY, B
    BRITES, F
    FRUCHART, JC
    DENEFLE, P
    DUVERGER, N
    CIRCULATION, 1994, 90 (04) : 81 - 81
  • [33] IMMUNONEPHELOMETRIC MEASUREMENT OF APOLIPOPROTEINS A-I AND A-II BY CONSECUTIVE ADDITION OF THE 2 ANTISERA TO A SINGLE SPECIMEN
    KUUSI, T
    PALOSUO, T
    EHNHOLM, C
    CLINICAL CHEMISTRY, 1984, 30 (07) : 1265 - 1266
  • [34] IMMUNOTURBIDIMETRIC METHOD FOR ROUTINE ASSAYS OF APOLIPOPROTEINS A-I, A-II, AND B IN NORMOLIPEMIC AND HYPERLIPEMIC SERUM
    SIEDEL, J
    SCHIEFER, S
    ROLLINGER, W
    ZIEGENHORN, J
    ANNALES DE BIOLOGIE CLINIQUE, 1985, 43 (04) : 676 - 676
  • [35] ELECTRO-IMMUNOASSAY AND RADIOIMMUNOASSAY FOR THE QUANTITATION OF HIGH-DENSITY APOLIPOPROTEINS A-I AND A-II
    MORDASINI, RC
    RIESEN, WF
    JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 1980, 18 (12): : 917 - 920
  • [36] AN OPTIMIZED ELECTROIMMUNOASSAY (EIA) TECHNIQUE FOR MEASURING SERUM APOLIPOPROTEINS-B, A, A-I AND A-II
    TALAMEH, Y
    NAITO, HK
    CLINICAL CHEMISTRY, 1984, 30 (06) : 993 - 993
  • [37] IMMUNOTURBIDIMETRIC METHOD FOR ROUTINE ASSAYS OF APOLIPOPROTEINS A-I, A-II, AND B IN NORMOLIPEMIC AND HYPERLIPEMIC SERUM
    SIEDEL, J
    SCHIEFER, S
    ZIEGENHORN, J
    CLINICAL CHEMISTRY, 1984, 30 (06) : 992 - 993
  • [38] Apolipoproteins A-I, A-II and B, lipoprotein(a) and the risk of ischaemic heart disease: the Caerphilly Study
    Sweetnam, PM
    Bolton, CH
    Downs, LG
    Durrington, PN
    Mackness, MI
    Elwood, PC
    Yarnell, JWG
    EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2000, 30 (11) : 947 - 956
  • [39] METABOLISM OF APOPROTEINS A-I AND A-II
    SHEPHERD, J
    PACKARD, C
    PATSCH, J
    GOTTO, AM
    TAUNTON, D
    CIRCULATION, 1977, 56 (04) : 5 - 5
  • [40] MECHANISM OF INHIBITION OF HEPATIC TRIGLYCERIDE LIPASE FROM HUMAN POST-HEPARIN PLASMA BY APOLIPOPROTEINS A-I AND A-II
    KUBO, M
    MATSUZAWA, Y
    YOKOYAMA, S
    TAJIMA, S
    ISHIKAWA, K
    YAMAMOTO, A
    TARUI, S
    JOURNAL OF BIOCHEMISTRY, 1982, 92 (03): : 865 - 870